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- 3D BBB Models
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Cell Lines for BBB Modeling
As the most basic unit in biological research, cells have been widely used as the vital study tool of basic research, drug screening, disease models and tissue engineering, thereby providing an important evidence for drug development and treatment. Although traditional animal models can more comprehensively simulate the complexity and specificity of human diseases, they are not easy to conduct a large number of repeated experiments due to the 3R principle and animal ethics issues. Compared with animal models, cell models, as a simple, fast and economical research tool, provide researchers with more opportunities to initially explore the mechanisms of disease and conduct large-scale drug screening.
BBB Models
The blood-brain barrier (BBB) refers to an extremely important histological barrier located in the intima of cerebral blood vessels, which composed of special cells including astrocytes, vascular endothelial cells and pericytes to generate neurovascular units and thereby involved in the regulation of neuronal activity. With the advancement of science and technology, more and more cell lines are used to construct in vitro BBB models. There are several cell lines that are commonly used for modeling the BBB.
- Brain capillary endothelial cell line (BCEC): The BCEC cell line is derived from brain capillary endothelial cells, which can form tight junctions and express various transporters and enzymes in the blood-brain barrier.
- Brain microvascular endothelial cell line (BMEC): The BMEC cell line is derived from brain microvascular endothelial cells and has been shown to exhibit multiple characteristics of the blood-brain barrier and can be used alone to create BBB models, such as human brain microvascular endothelial cell line (HBMEC).
- Astrocyte-derived cell lines: Astrocytes play a critical role in maintaining the integrity and function of the blood-brain barrier and are often used in combination with BCEC or BMEC cell lines to model the BBB.
- Pericyte-derived cell lines: Pericytes are another important cell type in the blood-brain barrier and are also commonly used in combination with BCEC or BMEC cell lines to model the BBB.
Ace Therapeutics provides hundreds of species, different types of cell lines that can be used alone or in combination to construct the models of BBB to study the behavior, structure and function of the blood-brain barrier, as well as for the development of drug delivery and treatment strategies for neurological diseases.
In addition to single cell lines, we provide multiple 3D coculture BBB models to more accurately represent the BBB, which typically involve a combination of endothelial cells (BCECs or BMECs) with other supporting cells such as astrocytes or pericytes.
Click on the Links to Access Our Cell Lines for BBB Modeling:
Cat.# Name Description Price BBM-C-152 Immortalized Human Brain Microvascular Endothelial Cells- Ras Immortalized Human Brain Microvascular Endothelial Cells- Ras from Ace Therapeutics are a specialized cell line developed by immortalizing primary cells using serial passaging and transduction with recombinant lentiviruses carrying the Ras gene. These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-153 Immortalized Human Brain Microvascular Endothelial Cells-P53 Immortalized Human Brain Microvascular Endothelial Cells-P53 from Ace Therapeutics are a specialized cell line developed by immortalizing primary cells using Tumor Protein 53 (p53) (Lentivirus). These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-154 Blood-Brain Barrier Immortalized hCMEC/D3 Cell Line The hCMEC/D3 BBB cell line is a model of the human blood-brain barrier (BBB) function. Blood-Brain Barrier Immortalized hCMEC/D3 Cell Line from Ace Therapeutics is derived from human temporal lobe microvessels isolated from tissue excised during surgery for control of epilepsy. These cells are a specialized cell line developed by immortalizing primary endothelial cells using the catalytic subunit of human telomerase (hTERT) and SV40 large T antigen. This brain microvascular endothelial cell line represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS).inquiry BBM-C-155 Blood-Brain Barrier Immortalized Rat Brain Vascular Endothelial Cells (GPNT) Blood-brain Barrier Immortalized Rat Brain Vascular Endothelial Cells (GPNT) from Ace Therapeutics are derived from microvessels in the cerebral cortex. The cells are immortalized by lipofection using an SV40 retroviral vector with a temperature-sensitive large T antigen (tsA58). GPNT exhibits a non-transformed phenotype. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-156 Immortalized CACO-2 Immortalized CACO-2 from Ace Therapeutics is a type of immortalized cell line derived from human colon carcinoma. Caco-2 cells not only are used to model the intestinal epithelial barrier but also can be used as a research barrier for the active transport of drugs. One of the most common ways Caco-2 cells are used in research is through permeability assays to study intestinal as well as blood-brain barrier permeability. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-157 Immortalized MDCK Immortalized Madin-Darby Canine Kidney cells (MDCK) from Ace Therapeutics are derived from kidney of normal female adult Cocker Spaniel canine. Similar to Immortalized CACO-2, it can be used to study the permeability of the blood-brain barrier. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-158 Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) from Ace Therapeutics is a specialized cell line developed by immortalizing primary cells using oncoprotein of murine Polyomavirus, Polyoma middle T antigen (PymT). These cell lines share principal features of the BBB in vivo, retaining the endothelial markers VE-Cadherin and PECAM-1, displaying tight junction-associated markers such as occludin, claudin-3, -5, -12, expressing the BBB marker protein Glut-1 and showing high electrical resistance, representing barrier properties. Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) can serve as a useful model for studies involving the differentiation and regulation of BBB. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-159 Immortalized Mouse Cerebellar Capillary Endothelial Cells (cerebEND) Immortalized Mouse Cerebellar Capillary Endothelial Cells (cerebEND) from Ace Therapeutics is a specialized cell line developed by immortalizing primary cells using oncoprotein of murine Polyomavirus, Polyoma middle T antigen (PymT). These cell lines share principal features of the BBB in vivo, retaining the endothelial markers VE-Cadherin and PECAM-1, displaying tight junction-associated markers such as occludin, claudin-3, -5, -12, expressing the BBB marker protein Glut-1 and showing high electrical resistance, representing barrier properties. Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) can serve as a useful model for studies involving the differentiation and regulation of BBB. Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) and Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) together represent important model systems for these different brain regions. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-160 Immortalized Mouse Brain Endothelial Cells - SV40 Immortalized Mouse Brain Endothelial Cells - SV40 from Ace Therapeutics are a specialized cell line developed by immortalizing primary mouse brain endothelial cells using SV40 Large T antigen. These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-161 Immortalized Rat Brain Microvascular Endothelial Cells - SV40 Immortalized Rat Brain Endothelial Cells - SV40 from Ace Therapeutics are a specialized cell line developed by immortalizing primary rat brain endothelial cells using SV40 Large T antigen. These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-162 Immortalized Bovine Brain Microvascular Endothelial Cells (BBrMEC) Immortalized Bovine Brain Microvascular Endothelial Cells (BBrMEC) from Ace Therapeutics are a specialized cell line developed by immortalizing primary bovine brain microvascular endothelial cells using T SV40 (pSV3-neo vector). Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-163 Immortalized Canine Brain Microvascular Endothelial Cells (CBrMEC) Immortalized Canine Brain Microvascular Endothelial Cells (CBrMEC) from Ace Therapeutics are a specialized cell line developed by immortalizing primary canine brain microvascular endothelial cells using T SV40 (pSV3-neo vector). Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-164 Immortalized Mouse Brain Microvascular Endothelial Cells - SV40T Immortalized Mouse Brain Microvascular Endothelial Cells - SV40T from Ace Therapeutics are a specialized cell line developed by immortalizing primary mouse brain microvascular endothelial using SV40 Large T antigen. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-165 Immortalized Mouse Brain Endothelial Cells - hTERT Immortalized Mouse Brain Endothelial Cells - hTERT from Ace Therapeutics are a specialized cell line developed by immortalizing primary mouse brain microvascular endothelial using recombinant lentiviruses carrying hTERT gene. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-166 Human iPSC-derived Brain Microvascular Endothelial Cells Human iPSC-derived Brain Microvascular Endothelial Cells (iBMECs) from Ace Therapeutics are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction condition. iBMEC cells are compact cellular structures when plated as a monolayer in culture and express standard biomarkers such as ECadherin and ZO-1 using immunohistochemistry methods and transporter biomarkers such as ABCB1 (P-gp), ABCG2, ABCE1, HIF1A, CLDN5, GLUT1, LAT1, MCT1, TJP1, PDGFbeta, OCLN, SLC25A3, TFR1, and SLC25A5 using RT/qPCR methods. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry All of our products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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