Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) from Ace Therapeutics is a specialized cell line developed by immortalizing primary cells using oncoprotein of murine Polyomavirus, Polyoma middle T antigen (PymT). These cell lines share principal features of the BBB in vivo, retaining the endothelial markers VE-Cadherin and PECAM-1, displaying tight junction-associated markers such as occludin, claudin-3, -5, -12, expressing the BBB marker protein Glut-1 and showing high electrical resistance, representing barrier properties. Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) can serve as a useful model for studies involving the differentiation and regulation of BBB. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Immortalized Mouse Brain Endothelial Cells - SV40 from Ace Therapeutics are a specialized cell line developed by immortalizing primary mouse brain endothelial cells using SV40 Large T antigen. These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Immortalized Bovine Brain Microvascular Endothelial Cells (BBrMEC) from Ace Therapeutics are a specialized cell line developed by immortalizing primary bovine brain microvascular endothelial cells using T SV40 (pSV3-neo vector). Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Immortalized Mouse Cerebellar Capillary Endothelial Cells (cerebEND) from Ace Therapeutics is a specialized cell line developed by immortalizing primary cells using oncoprotein of murine Polyomavirus, Polyoma middle T antigen (PymT). These cell lines share principal features of the BBB in vivo, retaining the endothelial markers VE-Cadherin and PECAM-1, displaying tight junction-associated markers such as occludin, claudin-3, -5, -12, expressing the BBB marker protein Glut-1 and showing high electrical resistance, representing barrier properties. Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) can serve as a useful model for studies involving the differentiation and regulation of BBB. Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) and Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) together represent important model systems for these different brain regions. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Immortalized Human Brain Microvascular Endothelial Cells- Ras from Ace Therapeutics are a specialized cell line developed by immortalizing primary cells using serial passaging and transduction with recombinant lentiviruses carrying the Ras gene. These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Immortalized CACO-2 from Ace Therapeutics is a type of immortalized cell line derived from human colon carcinoma. Caco-2 cells not only are used to model the intestinal epithelial barrier but also can be used as a research barrier for the active transport of drugs. One of the most common ways Caco-2 cells are used in research is through permeability assays to study intestinal as well as blood-brain barrier permeability. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Immortalized Rat Brain Endothelial Cells - SV40 from Ace Therapeutics are a specialized cell line developed by immortalizing primary rat brain endothelial cells using SV40 Large T antigen. These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Immortalized Canine Brain Microvascular Endothelial Cells (CBrMEC) from Ace Therapeutics are a specialized cell line developed by immortalizing primary canine brain microvascular endothelial cells using T SV40 (pSV3-neo vector). Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Immortalized Madin-Darby Canine Kidney cells (MDCK) from Ace Therapeutics are derived from kidney of normal female adult Cocker Spaniel canine. Similar to Immortalized CACO-2, it can be used to study the permeability of the blood-brain barrier. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Blood-brain Barrier Immortalized Rat Brain Vascular Endothelial Cells (GPNT) from Ace Therapeutics are derived from microvessels in the cerebral cortex. The cells are immortalized by lipofection using an SV40 retroviral vector with a temperature-sensitive large T antigen (tsA58). GPNT exhibits a non-transformed phenotype. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.