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- BBB DamageCell Death and ApoptosisNeuroinflammationNeuronal Damage and RepairOxidative StressVascular Mechanisms
- 3D BBB Models
- Organ-on-a-Chip BBB ModelsTranswell Models3D BBB Models Culture Substrate
- 3D HydrogelsCollagensAnmial BBB ModelsCell Lines for BBB Modeling
- Immortalized CellsPrimary CellsStem Cells
Stem Cells
Stem cells, also known as progenitor cells, are undifferentiated pluripotent cells with the ability to self-replicate and differentiate into different types of cells. They can self-renew and produce specific types of cells, such as muscle cells, nerve cells, heart cells, etc. Stem cells are widely used in medical research and treatment of various diseases due to their powerful regenerative capabilities.
Stem Cells for BBB Modeling
The blood-brain barrier (BBB) is a highly selective barrier that separates blood circulation from brain tissue. It plays a vital role in maintaining brain homeostasis by tightly regulating the movement of molecules, nutrients and cells between the blood and brain. Researchers create personalized BBB models by differentiating stem cells, such as induced pluripotent stem cells (iPSCs), into brain endothelial cells (BECs) to study individual differences in drug transport and disease mechanisms. In addition, researchers also use stem cells to design three-dimensional (3D) BBB models to study cell-cell interactions, barrier integrity, and drug penetration. Compared with traditional two-dimensional cell culture systems, 3D BBB models better simulate the complex structure and physiology of the BBB and can more realistically reflect the in vivo environment.
Overall, stem cells provide more possibilities for constructing BBB models, which helps researchers study the development, function, and dysfunction of the BBB in a controlled laboratory environment, thereby further elucidating neurodegenerative diseases, brain developmental disorders and potential mechanisms of drug delivery to the brain.
Ace Therapeutics provides a variety of, high-quality stem cells including iPSCs, embryonic stem cells, adult stem cells and iPSC-derived cells that can be used individually or in combination to create in vitro models of the BBB that mimic its physiological properties and functions. Our stem cells for BBB modeling are cultured and differentiation according to standardized procedures to ensure stable cell quality between different batc.
Additionally, we offer a variety of supplement media designed to balance and replenish specific cell types thus maximizing their life expectancy.
Cat.# Name Description Price BBM-C-166 Human iPSC-derived Brain Microvascular Endothelial Cells Human iPSC-derived Brain Microvascular Endothelial Cells (iBMECs) from Ace Therapeutics are derived from integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary induction condition. iBMEC cells are compact cellular structures when plated as a monolayer in culture and express standard biomarkers such as ECadherin and ZO-1 using immunohistochemistry methods and transporter biomarkers such as ABCB1 (P-gp), ABCG2, ABCE1, HIF1A, CLDN5, GLUT1, LAT1, MCT1, TJP1, PDGFbeta, OCLN, SLC25A3, TFR1, and SLC25A5 using RT/qPCR methods. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-167 Human Embryonic Hematopoietic Stem Cell Human Embryonic Hematopoietic Stem Cells (Plated cells are also available). 120 Population doublings or up to 12 passages. One million viable cells upon thawing of frozen cells, frozen vial of cells shipped in dry-ice. Cells are only guaranteed with the purchase of Ace Therapeutics‘s Media and Ace Therapeutics‘s Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.inquiry BBM-C-168 Human CD34+ Hematopoietic Stem Cell Human CD34+ Hematopoietic Stem Cell from Bone Marrow (HHSC-BM) or Liver (HHSC-L) contain CD34+ progenitor cells that differentiate into all the various blood cell types. Cells are only guaranteed with the purchase of Ace Therapeutics's Media and Ace Therapeutics's Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.inquiry BBM-C-169 Human Neural Stem Cells-cortex region Human Neural Stem Cells (HNSC) are self-renewing, generated throughout an adult’s life via neurogenesis. These multipotent adult stem cells generate the main phenotype of the nervous system, differentiating into neurons, astrocytes, and oligodendrocytes. Ace Therapeutics's Human Neural Stem Cells-cortex region are cells derived from the cortex region of human brain (single donor). They are cryopreserved at first passage. Our HNSC stain positive for β-tubulin III, GFAP and oligodendrocyte marker O4 when cultured in Human Neural Differentiation Medium for 10 days. Cells are only guaranteed with the purchase of Ace Therapeutics's Media and Ace Therapeutics's Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment.inquiry BBM-C-170 Human Cord Blood-CD34+ Hematopoietic Stem Cell Human Cord Blood-CD34+ Hematopoietic Stem Cell from Ace Therapeutics are isolated using positive magnetic isolation of CD34 from cord blood. CD34+ cells are targeted using uniform, superparamagnetic polymer beads coated with a primary monoclonal antibody specific for the CD34 membrane antigen predominantly expressed on human hematopoietic progenitor cells and endothelial progenitor cells. The isolated cells are poured off into a new tube and are cryogenically preserved.inquiry BBM-C-171 Human Cord Blood CD34/CD133+ Progenitor Cells CD34 is a well-known marker for primitive and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial progenitors. CD34+ progenitor cells are suitable for a series of studies for directed differentiation into more committed types of blood cells and endothelial lineages. Immediately after isolation, the freshly prepared CD34+ progenitor cells are cryopreserved using a serum-free freezing medium. The majority of CD133+ cells also express CD34. Cord blood CD133+ cells are isolated using direct positive immunomagnetic selection for CD133+ cells. Ace Therapeutics offers CD34+ progenitor cells and CD133 cells in a phenotypically undifferentiated state.inquiry BBM-C-172 Human Cord Blood Induced Pluripotent Stem Cell Cord blood iPSCs induced with non-viral EBNA1-based episomal vector pEV-SFFV-OS. All cell lines are tested to be free of any transgene vector insertion by PCR analysis and thus are authentic footprint-free iPSCs. These lines have all been passaged long-term (up to P30) without differentiation in optimized human iPSC culture media in the presence of inactivated REF or MEF feeder cells. These lines may have greater propensity to differentiate into Hematopoietic Stem Cells (HSCs) or other mesoderm cells due to epigenetic memory. Great cell model systems for any investigative research work including on iPSCs, HSCs, and disease modeling.Vector: pEV-SFFV-Oct4-2A-Sox2.inquiry BBM-C-173 Human Umbilical Cord Blood Mononuclear Cells Human Umbilical Cord Blood Mononuclear Cells are a mixture of various cell populations with a single nucleus, including hematopoietic and mesenchymal stem cells, endothelial progenitor cells, lymphocytes, and monocytes. They can be induced to differentiate into neuron cells, osteoblasts, and lymphocytes. Human Umbilical Cord Blood Mononuclear Cells from Ace Therapeutics are isolated from umbilical cord blood. Cells are isolated by density gradient separation and cryopreserved immediately to ensure the best viability. Human Umbilical Cord Blood Mononuclear Cells are quality tested with less than 2% red blood cells.inquiry BBM-C-174 Human Bone Marrow CD133+ Stem/Progenitor Cells Human Bone Marrow CD133+ Stem/Progenitor Cells from Ace Therapeutics are isolated using positive immunomagnetic cell separation procedures from bone marrow. Capable of a high level of proliferation and a wide range of differentiation, transplantation of bone marrow CD133+ cell lines has been shown to not only induced multilineage human hematopoiesis through the CD34+ lineage but also induce regeneration of cell lines in vitro in multiple locations by differentiating into endothelial cells, neural cells, hepatocytes, osteoblasts, and myocytes, with the list of tissue types this cell can differentiate into gradually increasing.inquiry BBM-C-175 Human Endothelial Progenitor Cell Human endothelial progenitor cells (EPCs) are circulating cells that exhibit a range of cell surface markers comparable to those found on vascular endothelial cells. They encompass primitive endothelial cells that possess enhanced angiogenic and vasculogenic characteristics. These primitive endothelial cells have the remarkable capacity to proliferate and undergo differentiation, ultimately maturing into functional endothelial cells. Human Endothelial Progenitor Cell from Ace Therapeutics can be expanded for up to 120 population doublings or 12 passages, providing ample material for experimentation. They express positive markers such as CD31, CD144, (VEGF)R2, CD146, CD73, CD105, and exhibit the uptake of acetylated low-density lipoprotein. Furthermore, AcceGen provides one million viable cells upon thawing of the frozen vial, which is shipped in dry ice to maintain cell integrity during transportation.inquiry BBM-C-176 Reprogrammed Human iPSC Line Human reprogrammed iPSC Cells established from endothelial progenitor cells that derived from peripheral blood.inquiry BBM-C-177 Human iPSC Line (Episomal, PBMC) Human iPSC Line (Episomal, PBMC) from Ace Therapeutics was derived from human mesenchymal stromal cells (MSCs) by ectopic expression of OCT4, SOX2, KLF4, and L-MYC genes using episomal plasmids.inquiry BBM-C-178 Human iPSC Line (Episomal, CB) The human iPSC line (episomal, CB) from Ace Therapeutics is a type of human induced pluripotent stem cell (iPSC) that was derived from human newborn cord blood (CB) mononuclear cells. The cells were reprogrammed into an embryonic-like pluripotent state using a method involving the introduction of OCT4, SOX2, KLF4, L-MYC, and Lin28 genes through episomal plasmids.inquiry BBM-C-179 Human iPSC-Derived Neural Stem Cell Human iPSC-Derived Neural Stem Cells are a homogeneous and multipotent population derived from control Human Induced Pluripotent Stem Cells.inquiry BBM-C-180 Human iPSC-derived Astrocytes Human iPSC-derived Astrocytes from Ace Therapeutics is derived from integration-free, induced pluripotent stem cells (iPSCs) under fully defined growth conditions using a completely reproducible process.inquiry All of our products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
Ace Therapeutics is a global leading provider of stroke research services. We are committed to accelerating progress in stroke research and drug development.
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