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- BBB DamageCell Death and ApoptosisNeuroinflammationNeuronal Damage and RepairOxidative StressVascular Mechanisms
- 3D BBB Models
- Organ-on-a-Chip BBB ModelsTranswell Models3D BBB Models Culture Substrate
- 3D HydrogelsCollagensAnmial BBB ModelsCell Lines for BBB Modeling
- Immortalized CellsPrimary CellsStem Cells
Immortalized Cells
The growth and metabolism of primary cells are in a dynamic balance of proliferation, differentiation, senescence and apoptosis. After experience limited culture, primary cells stop proliferating. A normal human primary cell can only replicate and divide 40 to 60 times before it is unable to divide and will age and die due to programmed cell death or apoptosis. Immortalized cells are derived from primary cells that have escaped normal aging and death and have been cultured for a long time. Cell immortalization strategies often use transgenic transfection technology to artificially introduce exogenous immortalization genes EBV, HPV E6/7 and SV40 T into target cells, or to knock out tumor suppressor genes, or to induce immortalization-related gene mutations to establish stable immortalized cell lines. Compared with normal cells that have a certain life cycle and eventually undergo aging and death, immortalized cells can survive for a long time and maintain a relatively stable physiological state, thereby providing researchers with more cell resources and serving as a good in vitro cell models contribute to the development of new treatment strategies and drugs.
Immortalized Cells for BBB Modeling
The blood-brain barrier (BBB) is a highly selective barrier that separates circulating blood from extracellular fluid in the brain. It plays a key role in maintaining brain homeostasis by regulating the movement of various substances in and out of the brain. As a highly stable and reproducible cell line, immortalized cells, including immortalized brain endothelial cells, immortalized astrocytes, and immortalized pericytes, have been widely used for the establishment of BBB models. Researchers can study the function and properties of the BBB and the ability of drugs to penetrate this barrier by using these cell lines. In addition, by using these immortalized cell lines, researchers can study changes in the BBB in various neurological diseases and find new treatment strategies. Overall, the immortalized cell BBB model provides a good platform for researchers to better understand and explore the interaction between the brain and the circulatory system, hleping support progress in the fields of neuroscience and drug development.
A commonly used immortalized cell line for BBB modeling is the hCMEC/D3 cell line, which are derived from human brain microvascular endothelial cells and exhibit many characteristics of the BBB, including high transendothelial resistance and expression of various tight junction proteins such as claudin-5 and occludin. Additionally, hCMEC/D3 cells retain the ability to actively transport molecules across the BBB, mimicking the in vivo function of the barrier...
Ace Therapeutics provides a variety of immortalized cells from humans and animals that can be used individually or in combination to create in vitro models of the BBB that mimic its physiological properties and functions. Our immortalized cells for BBB modeling are immortalize and cultured according to standardized procedures to ensure stable cell quality between different batches.
Additionally, we offer a variety of supplement media designed to balance and replenish specific cell types thus maximizing their life expectancy.
Cat.# Name Description Price BBM-C-151 Immortalized Human Brain Microvascular Endothelial Cells - SV40 Immortalized Human Brain Microvascular Endothelial Cells- SV40 from Ace Therapeutics are a specialized cell line developed by immortalizing primary cells using SV40 Large T antigen. These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-152 Immortalized Human Brain Microvascular Endothelial Cells- Ras Immortalized Human Brain Microvascular Endothelial Cells- Ras from Ace Therapeutics are a specialized cell line developed by immortalizing primary cells using serial passaging and transduction with recombinant lentiviruses carrying the Ras gene. These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-153 Immortalized Human Brain Microvascular Endothelial Cells-P53 Immortalized Human Brain Microvascular Endothelial Cells-P53 from Ace Therapeutics are a specialized cell line developed by immortalizing primary cells using Tumor Protein 53 (p53) (Lentivirus). These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-154 Blood-Brain Barrier Immortalized hCMEC/D3 Cell Line The hCMEC/D3 BBB cell line is a model of the human blood-brain barrier (BBB) function. Blood-Brain Barrier Immortalized hCMEC/D3 Cell Line from Ace Therapeutics is derived from human temporal lobe microvessels isolated from tissue excised during surgery for control of epilepsy. These cells are a specialized cell line developed by immortalizing primary endothelial cells using the catalytic subunit of human telomerase (hTERT) and SV40 large T antigen. This brain microvascular endothelial cell line represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS).inquiry BBM-C-155 Blood-Brain Barrier Immortalized Rat Brain Vascular Endothelial Cells (GPNT) Blood-brain Barrier Immortalized Rat Brain Vascular Endothelial Cells (GPNT) from Ace Therapeutics are derived from microvessels in the cerebral cortex. The cells are immortalized by lipofection using an SV40 retroviral vector with a temperature-sensitive large T antigen (tsA58). GPNT exhibits a non-transformed phenotype. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-156 Immortalized CACO-2 Immortalized CACO-2 from Ace Therapeutics is a type of immortalized cell line derived from human colon carcinoma. Caco-2 cells not only are used to model the intestinal epithelial barrier but also can be used as a research barrier for the active transport of drugs. One of the most common ways Caco-2 cells are used in research is through permeability assays to study intestinal as well as blood-brain barrier permeability. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-157 Immortalized MDCK Immortalized Madin-Darby Canine Kidney cells (MDCK) from Ace Therapeutics are derived from kidney of normal female adult Cocker Spaniel canine. Similar to Immortalized CACO-2, it can be used to study the permeability of the blood-brain barrier. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-158 Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) from Ace Therapeutics is a specialized cell line developed by immortalizing primary cells using oncoprotein of murine Polyomavirus, Polyoma middle T antigen (PymT). These cell lines share principal features of the BBB in vivo, retaining the endothelial markers VE-Cadherin and PECAM-1, displaying tight junction-associated markers such as occludin, claudin-3, -5, -12, expressing the BBB marker protein Glut-1 and showing high electrical resistance, representing barrier properties. Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) can serve as a useful model for studies involving the differentiation and regulation of BBB. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-159 Immortalized Mouse Cerebellar Capillary Endothelial Cells (cerebEND) Immortalized Mouse Cerebellar Capillary Endothelial Cells (cerebEND) from Ace Therapeutics is a specialized cell line developed by immortalizing primary cells using oncoprotein of murine Polyomavirus, Polyoma middle T antigen (PymT). These cell lines share principal features of the BBB in vivo, retaining the endothelial markers VE-Cadherin and PECAM-1, displaying tight junction-associated markers such as occludin, claudin-3, -5, -12, expressing the BBB marker protein Glut-1 and showing high electrical resistance, representing barrier properties. Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) can serve as a useful model for studies involving the differentiation and regulation of BBB. Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) and Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND) together represent important model systems for these different brain regions. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-160 Immortalized Mouse Brain Endothelial Cells - SV40 Immortalized Mouse Brain Endothelial Cells - SV40 from Ace Therapeutics are a specialized cell line developed by immortalizing primary mouse brain endothelial cells using SV40 Large T antigen. These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-161 Immortalized Rat Brain Microvascular Endothelial Cells - SV40 Immortalized Rat Brain Endothelial Cells - SV40 from Ace Therapeutics are a specialized cell line developed by immortalizing primary rat brain endothelial cells using SV40 Large T antigen. These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-162 Immortalized Bovine Brain Microvascular Endothelial Cells (BBrMEC) Immortalized Bovine Brain Microvascular Endothelial Cells (BBrMEC) from Ace Therapeutics are a specialized cell line developed by immortalizing primary bovine brain microvascular endothelial cells using T SV40 (pSV3-neo vector). Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-163 Immortalized Canine Brain Microvascular Endothelial Cells (CBrMEC) Immortalized Canine Brain Microvascular Endothelial Cells (CBrMEC) from Ace Therapeutics are a specialized cell line developed by immortalizing primary canine brain microvascular endothelial cells using T SV40 (pSV3-neo vector). Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-164 Immortalized Mouse Brain Microvascular Endothelial Cells - SV40T Immortalized Mouse Brain Microvascular Endothelial Cells - SV40T from Ace Therapeutics are a specialized cell line developed by immortalizing primary mouse brain microvascular endothelial using SV40 Large T antigen. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry BBM-C-165 Immortalized Mouse Brain Endothelial Cells - hTERT Immortalized Mouse Brain Endothelial Cells - hTERT from Ace Therapeutics are a specialized cell line developed by immortalizing primary mouse brain microvascular endothelial using recombinant lentiviruses carrying hTERT gene. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.inquiry All of our products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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