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Stains & Dyes for Stroke Research
Visual analysis of tissue and cellular imaging is critical to defining and understanding stroke mechanisms. Ace Therapeutics offers a range of histological stains and specialty stains to help our customers achieve optimal staining results. Our range of staining products and supporting protocols are widely used in stroke research to help researchers better understand the structure and function of the nervous system and advance stroke science. Additionally, we provides antifade fluorescence mounting media range of products to help you acquire the quality of images and minimal signal intensity loss during research.
Tag: IHC and Histological Stains, Fluorescence Probes, Ion Indicators, Proteins and Antibodies Labeling, Antifades & Mounting Media
For more detailed staining protocols, please access a specific product or contact our scientists.
Cat.# Name Size Description Price ASSD-101 Cresyl Violet Solution (1%) (AC101) 250 mL, 500 mL, This product is used to selectively stain Nissl substances in neurons on formalin-fixed, paraffin-embedded tissue. Commonly used to identify basic neuronal structures in brain and spinal cord tissue. Nissl granules are violet/purple, while the nuclei of glial cells and endothelial cells are slightly bluer (purple to dark blue) than Nissl granules. Controlling Tissue: cerebral cortexinquiry ASSD-102 DAPI (AC102) 10 mg, 50 mg DAPI is a blue fluorescent DNA stain that is cell-permeable at high concentrations. This product is commonly used as a nuclear and chromosomal counterstain. It is used preferentially for staining dead cells. DAPI is less effective as a live cell stain because it cannot efficiently cross the cell membrane of living cells. Therefore, higher concentrations may need to be used. Due to DAPI emitting blue, there is very little fluorescence overlap between yellow fluorescence, green fluorescent molecules (such as fluorescein and GFP), or red fluorescent stains (such as Texas Red). It is therefore suitable for multiplex assays. DAPI Staining Solution (1mg/mL) also available.inquiry ASSD-103 DAPI Staining Solution (1mg/mL) (AC103) 1 mL, 5 mL, DAPI is a blue fluorescent DNA stain which is cell permeant at high concentrations. This is a ready made 1mg/mL staining solution. It is recommended to dilute this solution 1:1000 with your desired buffer for a 1µg/mL working concentration. 0.1µg/mL-10µg/mL is the typical working concentration used in most applications. DAPI also available.inquiry ASSD-104 Hoechst 33258 (AC104) 25 mg, 100 mg Hoechst 33258, a blue fluorescent DNA stain, is commonly used in immunofluorescent work. It is frequently used as a nuclear stain to stain nuclei. Excited by UV light. The stain is also used as a substitute for DAPI and can be used on both live and fixed cells. As with other Hoechst stains, Hoechst 33258 binds to the AT-rich regions of the minor grove in DNA. It is less cell permeable but slightly more water soluble than the similar DNA stain Hoechst 33342. Unlike Hoechst 33342, Hoechst 33258 is not an apoptotic inducer.inquiry ASSD-105 Hoechst 33342 (AC105) 10 mg, 100 mg Hoechst 33342, a blue fluorescent DNA stain, is commonly used in fluorescent microscopy. It is frequently used as a nuclear stain to stain nuclei. It is excited by UV light. Hoechst 33342 is cell permeable and has greater cell permeability than Hoechst 33258. The stain can be used on both live and fixed cells and is often used as an alternative to DAPI. This product binds to the AT-rich regions of the minor grove in DNA which renders it specific for nuclear chromatin. Its fluorescent intensity depends on the DNA content, chromatin structure and the position of the cell within the cell cycle. Hoechst 33342 Staining Solution (10mg/ml) also available.inquiry ASSD-106 Hoechst 33342 Staining Solution (10mg/ml) (AC106) 5 mL, 10 mL 10mg/ml staining solution. Hoechst 33342, a blue fluorescent DNA stain, is commonly used in fluorescent microscopy and frequently used to stain nuclei and is cell permeable. Can be used on both live and fixed cells. Hoechst 33342 in solid powder form in 50mg and 100mg also available.inquiry ASSD-107 Modified Mayer's Hematoxylin (AC107) 125 mL, 250 mL, 2×250mL Hematoxylin is a nuclear stain with a deep blue-purple color. It is used as a counterstain for immunohistochemical or hybridization procedures that use colorimetric substrates (e.g. alkaline phosphatase or peroxidase). It is a component of one of the most widely used histology stains: the H&E stain (Hematoxylin and Eosin stain).inquiry ASSD-108 Neutral Red Solution (0.33%) (AC108) 50 mL, 100 mL Neutral Red is a Commonly used histological stain that stains lyosomes red. It can also be used as a counterstain with other dyes. It is frequently used as part of the neutral red uptake assay to provide a quantitative estimation of the number of viable cells in a culture.inquiry ASSD-109 Picro Sirius Red Solution (AC109) 250 mL The picro sirius red staining technique is frequently used to histologically stain collagen I and III fibers. It can be used to identify fibrillar collagen networks in tissue sections. When viewed under linear polarized light, collagens appear as green, red, orange or yellow fibers. Theoretical interpretation of staining/results: Polarized Light Microscopy: Type I (Thick fibers) Yellow-Orange Birefringence Type III (Thin fibers) Green Birefringence Light Microscopy: Collagen – Red Muscle Fibers – Yellow Cytoplasm - Yellowinquiry ASSD-110 Picro Sirius Red Stain Kit (AC110) 1 kit, 100 tests The picro sirius red staining technique is frequently used to histologically stain collagen I and III fibers. It can be used to identify fibrillar collagen networks in tissue sections. When viewed under linear polarized light, collagens appear as green, red, orange or yellow fibers. Components Picrosirius Red Solution 1 x 250 mL Acetic Acid Solution 2 x 250 mLinquiry ASSD-111 Propidium Iodide (AC111) 10 mg, 50 mg Propidium iodide (PI) is a widely used red-fluorescent intercalating agent that binds and labels nucleic acids. When bound to the nucleic acids, its fluorescence is enhanced 20- to 30-fold. Frequently used with Oxazole Yellow (YP1) when staining for apoptotic and necrotic cells as apoptotic cells remain impermeant to propidium iodide but permeable to Oxazole Yellow (YP1).inquiry ASSD-112 Propidium Iodide Staining Solution (1mg/ml) in water (AC112) 1 mL Propidium iodide (PI) is a widely used red-fluorescent intercalating agent that binds and labels nucleic acids. When bound to the nucleic acids, its fluorescence is enhanced 20- to 30-fold. Frequently used with Oxazole Yellow (YP1) when staining for apoptotic and necrotic cells as apoptotic cells remain impermeant to propidium iodide but permeable to Oxazole Yellow (YP1).inquiry ASSD-113 Thionin acetate salt (AC113) 5 g Cationic dye which is a histological stain and is used in the Nissl stain to label neuronal cell bodies.inquiry ASSD-114 Acridine Orange hydrochloride (AC114) 50 mg Acridine Orange hydrochloride is a cell-permeable fluorescent nucleic acid binding dye. It binds to both RNA and DNA producing fluorescence in a variety of colors. Inhibits mitosis. Displays anticancer properties under photon energy.inquiry ASSD-115 7-Amino-4-methylcoumarin (AC115) 100 mg, 500 mg, 1 g 7-Amino-4-methylcoumarin, a fluorochrome, is used for the trace determination of enzymes. And also suitable as a laser dye.inquiry All of our products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
Ace Therapeutics is a global leading provider of stroke research services. We are committed to accelerating progress in stroke research and drug development.
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