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Hoechst 33342 (AC105)
Cat.#: ASSD-105
Specifications
Description Hoechst 33342, a blue fluorescent DNA stain, is commonly used in fluorescent microscopy. It is frequently used as a nuclear stain to stain nuclei. It is excited by UV light. Hoechst 33342 is cell permeable and has greater cell permeability than Hoechst 33258. The stain can be used on both live and fixed cells and is often used as an alternative to DAPI.
This product binds to the AT-rich regions of the minor grove in DNA which renders it specific for nuclear chromatin. Its fluorescent intensity depends on the DNA content, chromatin structure and the position of the cell within the cell cycle.
Hoechst 33342 Staining Solution (10mg/ml) also available.Synonyms H33342, Bisbenzimide H 33342 Size 10 mg, 100 mg Applications • Counterstain
Hoechst 33342 is commonly used as a counterstain in fluorescent imaging.
• Cell cycle studies/Apoptosis
Hoechst 33342 can stain the condensed nuclei of apoptotic cells to allow the identification of chromatin condensation and fragmentation. It is commonly used with propidium iodide to distinguish normal/live apoptotic and dead cell populations. It can additionally be used in conjunction with arcidine orange (AO) to distinguish apoptotic cells. Incorporation of Brdu into DNA has a quenching effect on Hoechst fluorescence. Hoechst 33342 is also used in combination with BrdU to monitor cell cycle progression.
• Stem cells
Combination of the Hoechst 33342 stain with surface-marker phenotyping allows the characterization of a sub-population of stem cells termed the ‘side population’ (SP).Chemical Name 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole Molecule Weight 561.93 Molecular formula C27H28N6O.3HCl CAS Number 875756-97-1 PubChem identifier 1464 MDL Number MFCD00012678 Purity >98% Solubility Soluble in water, and in DMSO Storage Store at -20° C Shipping Shipped at 4°C Citation Guidance If you use our product in a scientific publication, please cite it as "Ace Therapeutics Cat.#". Click here to submit your paper's PubMed ID (PMID) to receive rewards. Related Products
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