Propidium Iodide (AC111)

Cat.#: ASSD-111

ASSD-111

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Specifications


Description Propidium iodide (PI) is a widely used red-fluorescent intercalating agent that binds and labels nucleic acids. When bound to the nucleic acids, its fluorescence is enhanced 20- to 30-fold. Frequently used with Oxazole Yellow (YP1) when staining for apoptotic and necrotic cells as apoptotic cells remain impermeant to propidium iodide but permeable to Oxazole Yellow (YP1).
Synonyms PI
Size 10 mg, 50 mg
Applications Flow cytometry, fluorescent microscopy and confocal laser scanning microscopy.

Propidium iodide is membrane impermeant and is therefore frequently used to selectively identify dead cells and is commonly used in flow cytometry to evaluate cell viability.
Chemical Name 2,7-Diamino-9-phenyl-10 (diethylaminopropyl)-phenanthridium iodide methiodide
Molecule Weight 668.39
Molecular formula C27H34I2N4
CAS Number 25535-16-4
PubChem identifier 104981
MDL Number MFCD00011921
Purity >97%
Solubility Soluble in water (5 mM)
Form Purple solid
Excitation 535 nm
Emission 617 nm
Storage Store at 4° C
Shipping Shipped at 4°C

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