Cat.#: ASSD-104
| Description | Hoechst 33258, a blue fluorescent DNA stain, is commonly used in immunofluorescent work. It is frequently used as a nuclear stain to stain nuclei. Excited by UV light. The stain is also used as a substitute for DAPI and can be used on both live and fixed cells. As with other Hoechst stains, Hoechst 33258 binds to the AT-rich regions of the minor grove in DNA. It is less cell permeable but slightly more water soluble than the similar DNA stain Hoechst 33342. Unlike Hoechst 33342, Hoechst 33258 is not an apoptotic inducer. |
| Synonyms | bisBenzimide; HO 33258; Hoechst-33258 |
| Size | 25 mg, 100 mg |
| Applications | Hoechst 33258 is suitable for a variety of applications as there is little fluorescent overlap with other used fluorophores/fluorescent proteins that emit in the green/red range. The stain is frequently used as a nuclear counterstain, for cell cycle analysis and to distinguish apoptotic cells. As this stain does not induce apoptosis, it may be preferential to Hoecsht 33342 for identification and isolation of the stem cell side population. |
| Chemical Name | Bisbenzimidazole trihydrochloride |
| Molecule Weight | 533.88 |
| Molecular formula | C25H24N6O 3HCl |
| CAS Number | 23491-45-4 |
| PubChem identifier | 31953 |
| MDL Number | MFCD00012679 |
| Purity | >95% |
| Solubility | Soluble in water (100 mM), and in DMSO (20 mM) |
| Storage | Store at 4° C (desiccate) |
| Shipping | Shipped at 4°C |
| Cat.# | Name | Size | Price |
|---|---|---|---|
| ASSD-213 | Fluorescein 6-Maleimide (AC213) | 25 mg, 250 mg | inquiry |
| ASSD-189 | 7-Hydroxycoumarin-3-carboxylic acid (AC189) | 100 mg | inquiry |
| ASSD-178 | 5-(Iodoacetamido)fluorescein (AC178) | 25 mg, 100 mg, 250 mg | inquiry |
| ASSD-144 | Oxazole Yellow Iodide (AC144) | 1 mg | inquiry |
| ASSD-207 | NBD-X acid (AC207) | 1 g | inquiry |
| ASSD-102 | DAPI (AC102) | 10 mg, 50 mg | inquiry |
| ASSD-103 | DAPI Staining Solution (1mg/mL) (AC103) | 1 mL, 5 mL, | inquiry |
| ASSD-101 | Cresyl Violet Solution (1%) (AC101) | 250 mL, 500 mL, | inquiry |
| ASSD-151 | Acid Fuchsin (AC151) | 25 g | inquiry |
| ASSD-168 | Indo-1 AM (AC168) | 1 mg | inquiry |
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