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DAPI (AC102)
Cat.#: ASSD-102
Specifications
Description DAPI is a blue fluorescent DNA stain that is cell-permeable at high concentrations. This product is commonly used as a nuclear and chromosomal counterstain. It is used preferentially for staining dead cells. DAPI is less effective as a live cell stain because it cannot efficiently cross the cell membrane of living cells. Therefore, higher concentrations may need to be used.
Due to DAPI emitting blue, there is very little fluorescence overlap between yellow fluorescence, green fluorescent molecules (such as fluorescein and GFP), or red fluorescent stains (such as Texas Red). It is therefore suitable for multiplex assays.
DAPI Staining Solution (1mg/mL) also available.Size 10 mg, 50 mg Applications The product is commonly used for cell imaging, cell counting, cell sorting (based on DNA content), apoptosis analysis, and HCA (high content analysis). Chemical Name 4',6-Diamidino-2-phenylindole dihydrochloride Molecule Weight 350.24 Molecular formula C16H15N5.2HCl CAS Number 28718-90-3 PubChem identifier 160166 MDL Number MFCD00012681 Purity >98% Solubility Soluble in water (10mg/ml, gentle warming), and in methanol Excitation 340/360 nm (for ds-DNA) Emission 488 460 nm (for ds-DNA) Storage Store at -20° C Shipping Shipped at 4°C Important When using this product, cells must be permeabilized and/or fixed so that DAPI can enter the cells and bind to the DNA. Citation Guidance If you use our product in a scientific publication, please cite it as "Ace Therapeutics Cat.#". Click here to submit your paper's PubMed ID (PMID) to receive rewards. Related Products
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