Cat.#: ASSD-106
| Description | 10mg/ml staining solution. Hoechst 33342, a blue fluorescent DNA stain, is commonly used in fluorescent microscopy and frequently used to stain nuclei and is cell permeable. Can be used on both live and fixed cells. Hoechst 33342 in solid powder form in 50mg and 100mg also available. |
| Synonyms | H33342, Bisbenzimide H 33342 |
| Size | 5 mL, 10 mL |
| Applications | • Counterstain Hoechst 33342 is commonly used as a counterstain in fluorescent imaging. • Cell cycle studies/Apoptosis Hoechst 33342 can stain the condensed nuclei of apoptotic cells to allow the identification of chromatin condensation and fragmentation. It is commonly used with propidium iodide to distinguish normal/live apoptotic and dead cell populations. It can additionally be used in conjunction with arcidine orange (AO) to distinguish apoptotic cells. Incorporation of Brdu into DNA has a quenching effect on Hoechst fluorescence. Hoechst 33342 is also used in combination with BrdU to monitor cell cycle progression. • Stem cells Combination of the Hoechst 33342 stain with surface-marker phenotyping allows the characterization of a sub-population of stem cells termed the ‘side population’ (SP). |
| Chemical Name | 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole |
| Molecule Weight | 561.93 |
| Molecular formula | C27H28N6O.3HCl |
| CAS Number | 875756-97-1 |
| Solubility | 10mg/ml |
| Storage | Store at -20° C |
| Shipping | Shipped at 4°C |
| Cat.# | Name | Size | Price |
|---|---|---|---|
| ASSD-116 | Annexin V-FITC Apoptosis Staining / Detection Kit (AC116) | 1 kit, 100 tests | inquiry |
| ASSD-180 | Ru(bpy)2(mcbpy-O-Su-ester)(PF6)2 (AC180) | 1 mg | inquiry |
| ASSD-142 | Methoxy-X04 (AC142) | 10 mg, 50 mg | inquiry |
| ASSD-189 | 7-Hydroxycoumarin-3-carboxylic acid (AC189) | 100 mg | inquiry |
| ASSD-143 | Fluorescently labeled Muscimol TMR-X conjugate (AC143) | 1mg | inquiry |
| ASSD-136 | LUF7445/A2A antagonist (AC136) | 50 μg | inquiry |
| ASSD-141 | MTT (AC141) | 500 mg, 1 g | inquiry |
| ASSD-173 | FITC-Dextran 70 (AC173) | 100 mg, 500 mg | inquiry |
| ASSD-140 | A2B covalent ligand (AC140) | 50 μg | inquiry |
| ASSD-167 | Fura-2 AM (Cell permeant) (AC167) | 1 mg | inquiry |
Ace Therapeutics is a global leading provider of stroke research services. We are committed to accelerating progress in stroke research and drug development.