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Hoechst 33342 Staining Solution (10mg/ml) (AC106)
Cat.#: ASSD-106
Specifications
Description 10mg/ml staining solution. Hoechst 33342, a blue fluorescent DNA stain, is commonly used in fluorescent microscopy and frequently used to stain nuclei and is cell permeable. Can be used on both live and fixed cells.
Hoechst 33342 in solid powder form in 50mg and 100mg also available.Synonyms H33342, Bisbenzimide H 33342 Size 5 mL, 10 mL Applications • Counterstain
Hoechst 33342 is commonly used as a counterstain in fluorescent imaging.
• Cell cycle studies/Apoptosis
Hoechst 33342 can stain the condensed nuclei of apoptotic cells to allow the identification of chromatin condensation and fragmentation. It is commonly used with propidium iodide to distinguish normal/live apoptotic and dead cell populations. It can additionally be used in conjunction with arcidine orange (AO) to distinguish apoptotic cells. Incorporation of Brdu into DNA has a quenching effect on Hoechst fluorescence. Hoechst 33342 is also used in combination with BrdU to monitor cell cycle progression.
• Stem cells
Combination of the Hoechst 33342 stain with surface-marker phenotyping allows the characterization of a sub-population of stem cells termed the ‘side population’ (SP).Chemical Name 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole Molecule Weight 561.93 Molecular formula C27H28N6O.3HCl CAS Number 875756-97-1 Solubility 10mg/ml Storage Store at -20° C Shipping Shipped at 4°C Citation Guidance If you use our product in a scientific publication, please cite it as "Ace Therapeutics Cat.#". Click here to submit your paper's PubMed ID (PMID) to receive rewards. Related Products
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