Cat.#: ASSD-106
| Description | 10mg/ml staining solution. Hoechst 33342, a blue fluorescent DNA stain, is commonly used in fluorescent microscopy and frequently used to stain nuclei and is cell permeable. Can be used on both live and fixed cells. Hoechst 33342 in solid powder form in 50mg and 100mg also available. |
| Synonyms | H33342, Bisbenzimide H 33342 |
| Size | 5 mL, 10 mL |
| Applications | • Counterstain Hoechst 33342 is commonly used as a counterstain in fluorescent imaging. • Cell cycle studies/Apoptosis Hoechst 33342 can stain the condensed nuclei of apoptotic cells to allow the identification of chromatin condensation and fragmentation. It is commonly used with propidium iodide to distinguish normal/live apoptotic and dead cell populations. It can additionally be used in conjunction with arcidine orange (AO) to distinguish apoptotic cells. Incorporation of Brdu into DNA has a quenching effect on Hoechst fluorescence. Hoechst 33342 is also used in combination with BrdU to monitor cell cycle progression. • Stem cells Combination of the Hoechst 33342 stain with surface-marker phenotyping allows the characterization of a sub-population of stem cells termed the ‘side population’ (SP). |
| Chemical Name | 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole |
| Molecule Weight | 561.93 |
| Molecular formula | C27H28N6O.3HCl |
| CAS Number | 875756-97-1 |
| Solubility | 10mg/ml |
| Storage | Store at -20° C |
| Shipping | Shipped at 4°C |
| Cat.# | Name | Size | Price |
|---|---|---|---|
| ASSD-110 | Picro Sirius Red Stain Kit (AC110) | 1 kit, 100 tests | inquiry |
| ASSD-181 | Nitrofluorescein, Isomer 1 (AC181) | 1 g | inquiry |
| ASSD-222 | Antifade Fluorescence Mounting Medium with Propidium Iodide (hard set) (AC222) | 10 mL | inquiry |
| ASSD-206 | Eosine-5-isothiocyanate (AC206) | 50 mg | inquiry |
| ASSD-202 | 5(6)-Carboxytetramethylrhodamine (AC202) | 100 mg, 250 mg | inquiry |
| ASSD-190 | Chrysamine G Disodium Salt (AC190) | 10 mg, 25 mg | inquiry |
| ASSD-209 | Tetramethylrhodamine isothiocyanate Isomer R (AC209) | 5 mg, 10 mg | inquiry |
| ASSD-104 | Hoechst 33258 (AC104) | 25 mg, 100 mg | inquiry |
| ASSD-214 | 8-Aminopyrene-1,3,6-trisulfonic acid trisodium salt (AC214) | 10 mg | inquiry |
| ASSD-132 | Congo Red (AC132) | 100 mg, 1g | inquiry |
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