Ace Therapeutics has scientists committed to discovery and innovation, and we are always working to find new ways to streamline life science research. Whether you trust us for oxidative stress assays, gene expression analysis or protein analysis, we can help you with experimental design, assay protocols and data analysis. We are willing to continually develop new assays to advance the cellular and molecular biology associated with mental illness.

Introduction of Oxidative Stress Assays in Psychiatry

Oxidative stress is caused by excess free radicals produced by cellular metabolic stress and impaired antioxidant defense systems and is known to cause membrane dysfunction associated with the pathophysiology of schizophrenia. Oxidative stress mechanisms have been extensively studied in schizophrenia, employing various areas of oxidative research, including oxidative biomarker assays, psychopharmacology, and clinical studies of antioxidants. Advances in new technologies and increased understanding will allow us to develop new therapies for clinical symptoms and identify preventive mechanisms to halt the transition to schizophrenia in populations at high risk for mental illness.

Fig. 1 Pharmacological modulation of the redox state in psychiatric disorders. (Rossetti AC, et al., 2020)

Oxidative Stress Assay Services

• DNA Damage and Repair in Psychiatry

We offer 8-OHG RNA Damage Assays, 8-OHdG DNA Damage Assays, AP Sites Quantitation, Comet Assays, DNA Double-Strand Break Assays, Poly (ADP-Ribose) ELISA, and UV Induced DNA Damage Assays. Our assay services can help you study how altered DNA repair is involved in the pathogenesis of depression.

• Lipid Peroxidation in Psychiatry

We offer reliable assays to detect the most common lipid peroxidation by-products: malondialdehyde (MDA), 4-hydroxynonenal (4-HNE) and 8-iso-prostaglandin F2-alpha (8-isoprostane). We support researchers studying the link between lipid peroxidation and disorders such as schizophrenia, bipolar disorder and major depression.

• Protein Oxidation or Nitration in Psychiatry

We offer universal assays to detect protein oxidation/nitration, including advanced glycosylation end product assays, advanced oxidized protein product (AOPP) assays, oxidized/modified human lipoprotein assays, protein carbamylation assays, protein carbonyl assays, protein nitration assays, nitrotyrosine assays, and S-glutathione protein adduct assays. Our services can assist researchers in studying the role of protein oxidation, lipid peroxidation and DNA oxidative damage in psychiatric disorders.

• Reactive Oxygen Species (ROS) Assays in Psychiatry

We offer two general assays for the detection of reactive oxygen species: an intracellular ROS assay for intact cells and an in vitro ROS assay for cell lysates, tissue homogenates, blood or urine. We are committed to helping you analyze the role of ROS and oxidative damage in the pathophysiology of schizophrenia.

• Antioxidant Assays in Antipsychotic Therapy

We offer assays to measure the activity of specific antioxidants, including catalase, superoxide dismutase, and glutathione. In addition, we can measure the antioxidant capacity of certain biomolecules (FRAP, ORAC, HORAC and TAC) as well as test exogenous antioxidant compounds in a cell-based physiological setting.

• Oxidase Activity Assays in Psychiatry

We offer oxidase assays, peroxidase/hydrogen peroxide assays, myeloperoxidase chloride activity assays, and polyamine oxidase assays. We are committed to helping you help you study the relationship between oxidase or peroxidase dysfunction and psychiatric disorders such as depression, substance abuse and schizophrenia.

Our Advantages

• Reliable and reproducible results
• Enables high-throughput screening
• Testing according to your requirements
• Projects are designed and handled by experienced scientists.
• Short turnaround time
• Competitive pricing

Ace Therapeutics is committed to providing biomedical researchers with the best services available for analyzing lipid peroxidation reactions to help accelerate your drug development process for psychiatric disorders. If you are interested in our services, please feel free to make an inquiry.

Reference

1. Rossetti AC, et al. Oxidation-reduction mechanisms in psychiatric disorders: A novel target for pharmacological intervention. Pharmacol Ther. 2020, 210:107520.