Ace Therapeutics has an advanced technology platform for antipsychotic drug development, and we are committed to providing oxidative stress testing services for psychiatric-based research to help researchers successfully achieve their research goals.

Introduction of Antioxidant Assays in Antipsychotic Therapy

A growing body of evidence suggests that an imbalance between oxidative stress and antioxidant defense systems may be associated with the development of neuropsychiatric disorders such as depression and anxiety disorders. Currently, major depression and anxiety are associated with a reduced total antioxidant status and an activated oxidative stress (OS) pathway. Antioxidants may further protect the brain from neuronal damage caused by oxidative or nitrosative stressors by scavenging free radicals and inhibiting OS pathways to remove reactive oxygen species (ROS) and reactive nitrogen species (RNS), which is expected to lead to relief of depression or anxiety symptoms. A functional understanding of the relationship between oxidative stress and depression and anxiety may pave the way for the discovery of new targets for the treatment of neuropsychiatric disorders.

Fig. 1 Oxidative stress in the mental disorders under study. (Kotzaeroglou A, et al., 2022)

Antioxidant Assay Services

Ace Therapeutics offers assays to measure the activity of specific antioxidants: catalase, superoxide dismutase and glutathione. In addition, we can measure the antioxidant capacity of certain biomolecules (FRAP, ORAC, HORAC and TAC) as well as test exogenous antioxidant compounds in a cell-based physiological environment.

Specifically, we provide testing services including but not limited to

• Antioxidant capacity assay, including ferric reducing antioxidant capacity (FRAP) assay, hydroxyl radical antioxidant capacity (HORAC) assay, oxygen radical antioxidant capacity (ORAC) assay, Trolox equivalent antioxidant capacity (TEAC) assay and total antioxidant capacity (TAC) assay
• Antioxidant enzyme activity assay, including catalase activity assay and superoxide dismutase (SOD) activity assay
• Ascorbic acid assay (FRASC)
• Cell-based exogenous antioxidant assay
• Chitosan assay
• Flavonoid assay
• Glutathione assay, including glutathione reductase assay and total glutathione assay
• Hydrogen sulfide gas assay
• Total thiol assay

Sample Request

• FRAP assay: serum, plasma, lysates, biological fluids, and purified food or drug extracts
• HORAC and ORAC assay: plasma, cell fractions, tissue lysates, solid and aqueous nutrient samples
• TEAC and TAC assay: plasma, serum, urine, cell lysates, tissue homogenates and food extracts
• Catalase activity assay: plasma, serum, cell lysates or tissue homogenates
• SOD activity assay: plasma, serum, cell or tissue
• FRASC assay: serum, plasma, urine, saliva, tissue homogenates, cell extracts and purified food or drug extracts
• Chitosan assay: serum, urine or shell extracts
• Flavonoid assay: for serum, plasma, tissue extracts and food extracts
• Glutathione assay: serum, plasma, saliva, urine, tissue extracts and mammalian or plant cell lysates
• Hydrogen sulfide gas assay: samples containing hydrogen sulfide donors, such as enzymes, cells or tissues
• Total thiol assay: serum, plasma, biological samples and lysates

What Can We Help You Achieve in Psychiatry?

• Investigating the Mechanisms of Antioxidant Defense Imbalance in Mental Illness
• Development of antioxidant therapies for schizophrenia
• Study of antioxidant mechanism of action of antipsychotic drugs
• Development of potential targets for psychiatric disorders
• Analysis of the causal relationship between circulating antioxidant levels and major psychiatric disorders including AD, MDD, BIP, SCZ, PTSD and OCD

Ace Therapeutics is committed to supporting customers worldwide in making breakthrough scientific discoveries to accelerate new drug discovery and scientific diagnosis and treatment of anti-psychiatric disorders. Our high-performance scientific instruments and high-value solutions enable you to make great advances in psychiatric pathology. Let us know your project needs and our scientists will provide you with a full range of services from solutions to reporting. Please feel free to make an inquiry with any questions you may have.

Reference

1. Kotzaeroglou A, Tsamesidis I. The Role of Equilibrium between Free Radicals and Antioxidants in Depression and Bipolar Disorder. Medicines (Basel). 2022, 9(11):57.