Porcine Brain Microvascular Endothelial Cells from Ace Therapeutics are isolated from healthy porcine brain tissue. Porcine Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with a gelatin-based solution for 0.5 hours and incubated in Ace Therapeutics’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

C57BL/6 mouse brain endothelial cells (MBREC) from Ace Therapeutics are isolated from the brain tissue of pathogen-free laboratory mice. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Mouse Astrocytes-hippocampal from CD1 from Ace Therapeutics are isolated from postnatal day 2 mouse hippocampus and are cryopreserved as primary cultures. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.

Rat Astrocytes (RA) from Ace Therapeutics are isolated from postnatal day 2 rat brains and are cryopreserved as primary cultures. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.

Human Brain Microvascular Endothelial Cells (HBMECs), derived from the human brain, are a specific type of brain endothelial cells that play a crucial role in forming the blood-brain barrier (BBB). They prevent the entry of soluble and cellular substances from the blood into the brain and protect it against toxins and immune cells. These brain microvascular endothelial cells have the ability to display cell adhesion molecules on their surface, which control the migration of leukocytes into the brain. Nevertheless, when there are host-pathogen interactions outside the cells, it can activate calcium signaling within the endothelial cells, leading to the penetration of pathogens through the BBB. Human Brain Microvascular Endothelial Cells from Ace Therapeutics are isolated from normal human brain cortical tissue. Each vial contains at least 5×10^5 cells per ml and is delivered frozen.

Mouse Brain Vascular Pericytes (MBVP) from Ace Therapeutics are isolated from adult mouse brains and cryopreserved at P2. MBVP express Neural/glial antigen 2 (NG2) and α-smooth muscle actin. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Characterization: 1. Cell Activity; – Recovery Viability ≥ 80%. 2. Cell Purity; – GFAP-positive cells ≥ 70%. – β-tubulin III- or doublecortin- (Dcx-) positive cells ≤ 10%. – GalC-positive cells ≤ 10%.

Rabbit Cerebrovascular Pericytes from Ace Therapeutics are isolated from the brain tissue of a pathogen-free New Zealand White Rabbit. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

BALB/c Mouse Brain Microvascular Endothelial Cells from Ace Therapeutics are isolated from the brain tissue of pathogen-free laboratory mice. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Bovine Brain Microvascular Endothelial Cells (BBMVEC) from Ace Therapeutics are isolated from microvessels of the bovine brain. The cells are cryopreserved at the second passage.