3D BBB Models

In vitro blood-brain barrier (BBB) models can reproduce BBB behavior in a controlled environment to better study how the barrier reacts to stress events and drug transport. Ace Therapeutics offers dozens of in vitro 3D BBB models for you to choose from.

Why do You Need In Vitro 3D BBB Models?

The blood-brain barrier is a complex physiological barrier that separates the brain from circulating blood. Compared to conventional 2D cell cultures, in vitro 3D BBB models provide a controlled and reproducible platform that allows the structure, function, and permeability of the BBB to be studied in a more physiologically relevant manner. By utilizing 3D models, researchers can mimic the intricate architecture and cellular interactions seen in the in vivo BBB. These models typically consist of endothelial cells, which form the BBB's primary barrier, along with other cell types such as astrocytes and pericytes that play crucial roles in maintaining BBB integrity and function.

Additionally, another of the key advantages of 3D BBB models is their ability to accurately mimic the physical and mechanical properties of the BBB microenvironment, which enables researchers to study the effects of factors such as shear stress and fluid flow, which are known to influence BBB development and function.

In terms of functionality, 3D BBB models can replicate the selective permeability of the BBB observed in vivo, which allows for the assessment of the permeability characteristics of the BBB by measuring the transendothelial electrical resistance (TEER) and the permeability coefficient of various compounds across the model.

Click on the Links to Access Our 3D BBB Models:

Transwell Models Organ-on-a-Chip BBB Models

Cat.#	Name	Description	Price
BBM-M-001	Human Blood-Brain Barrier Model	Human Blood-Brain Barrier Model from Ace Therapeutics is developed by culturing brain endothelial cells with pericytes and astrocytes layered in an insert. The model mimics transport properties of the Blood Brain Barrier (BBB) due to the formation of tight junctions, higher expression of specific carriers, or great cell viability. Compared with the monoculture model, our 3D in vivo model of the BBB can truly mimic the BBB by enhancing the polarization of endothelial cells, facilitating the formation of tight junctions, promoting essential endothelial cell-to-cell contact for barrier development, and preventing the dilution of secreted neurotrophic factors.	inquiry
BBM-M-002	Human Blood-Brain Barrier Modeling Kit	Human Blood-Brain Barrier Modeling Kit from Ace Therapeutics co-cultures endothelial cells, pericytes, and astrocytes in a collagen I matrix with a defined serum-free medium to replicate intercellular interactions at the BBB. Our Models come with media, and you can also order additional media. We provide a series of 3D BBB model culture substrates, such as collagens and hydrogels, for you.	inquiry
BBM-M-003	3D Human Blood-Brain Barrier Modeling Kit with HBMEC	3D Human Blood-Brain Barrier Modeling Kit with HBMEC from Ace Therapeutics is developed by culturing human brain microvascular endothelial cells (HBMEC), brain vascular pericytes, and astrocytes at a 1:1:1 ratio. Our Models come with media, and you can also order additional media. We provide a series of 3D BBB model culture substrates, such as collagens and hydrogels, for you.	inquiry
BBM-M-009	3D Blood Brain Barrier Model (TEER configuration)	3D Blood Brain Barrier Model recreates the in vivo microenvironment by mimicking the histology of brain tissue cells that communicate with BBB epithelial endothelial cells. The IMN2 TEER option allows you to accurately measure resistance, providing a non-invasive method to monitor tight junctions in real-time.	inquiry
BBM-M-010	3D Blood-Brain Barrier Model (IMN2 linear)	3D Blood Brain Barrier Model recreates the in vivo microenvironment by mimicking the histology of brain tissue cells that communicate with BBB epithelial endothelial cells.	inquiry
BBM-M-011	3D Blood-Brain Barrier Model (IMN2 radial)	3D Blood Brain Barrier Model recreates the in vivo microenvironment by mimicking the histology of brain tissue cells that communicate with BBB epithelial endothelial cells.	inquiry

All of our products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.