3D BBB Models

In vitro blood-brain barrier (BBB) models can reproduce BBB behavior in a controlled environment to better study how the barrier reacts to stress events and drug transport. Ace Therapeutics offers dozens of in vitro 3D BBB models for you to choose from.

Why do You Need In Vitro 3D BBB Models?

The blood-brain barrier is a complex physiological barrier that separates the brain from circulating blood. Compared to conventional 2D cell cultures, in vitro 3D BBB models provide a controlled and reproducible platform that allows the structure, function, and permeability of the BBB to be studied in a more physiologically relevant manner. By utilizing 3D models, researchers can mimic the intricate architecture and cellular interactions seen in the in vivo BBB. These models typically consist of endothelial cells, which form the BBB's primary barrier, along with other cell types such as astrocytes and pericytes that play crucial roles in maintaining BBB integrity and function.

Additionally, another of the key advantages of 3D BBB models is their ability to accurately mimic the physical and mechanical properties of the BBB microenvironment, which enables researchers to study the effects of factors such as shear stress and fluid flow, which are known to influence BBB development and function.

In terms of functionality, 3D BBB models can replicate the selective permeability of the BBB observed in vivo, which allows for the assessment of the permeability characteristics of the BBB by measuring the transendothelial electrical resistance (TEER) and the permeability coefficient of various compounds across the model.

Click on the Links to Access Our 3D BBB Models:

Transwell Models Organ-on-a-Chip BBB Models

Cat.#	Name	Description	Price
BBM-M-001	Human Blood-Brain Barrier Model	Human Blood-Brain Barrier Model from Ace Therapeutics is developed by culturing brain endothelial cells with pericytes and astrocytes layered in an insert. The model mimics transport properties of the Blood Brain Barrier (BBB) due to the formation of tight junctions, higher expression of specific carriers, or great cell viability. Compared with the monoculture model, our 3D in vivo model of the BBB can truly mimic the BBB by enhancing the polarization of endothelial cells, facilitating the formation of tight junctions, promoting essential endothelial cell-to-cell contact for barrier development, and preventing the dilution of secreted neurotrophic factors.	inquiry
BBM-M-002	Human Blood-Brain Barrier Modeling Kit	Human Blood-Brain Barrier Modeling Kit from Ace Therapeutics co-cultures endothelial cells, pericytes, and astrocytes in a collagen I matrix with a defined serum-free medium to replicate intercellular interactions at the BBB. Our Models come with media, and you can also order additional media. We provide a series of 3D BBB model culture substrates, such as collagens and hydrogels, for you.	inquiry
BBM-M-003	3D Human Blood-Brain Barrier Modeling Kit with HBMEC	3D Human Blood-Brain Barrier Modeling Kit with HBMEC from Ace Therapeutics is developed by culturing human brain microvascular endothelial cells (HBMEC), brain vascular pericytes, and astrocytes at a 1:1:1 ratio. Our Models come with media, and you can also order additional media. We provide a series of 3D BBB model culture substrates, such as collagens and hydrogels, for you.	inquiry
BBM-M-004	Caco-2 Blood Brain Barrier Modeling Kit	Caco-2 Blood Brain Barrier Modeling Kit from Ace Therapeutics is developed by seeding with 21 days of differentiated and polarized Caco-2 cells on the Transwell insert plates. Our Models come with media, and you can also order additional media. We provide a series of 3D organoid BBB model culture substrates, such as collagens and hydrogels, for you.	inquiry
BBM-M-005	MDCKII Blood Brain Barrier Modeling Kit	MDCKII Blood Brain Barrier Modeling Kit from Ace Therapeutics is developed by seeded with 21 days differentiated and polarized Caco-2 and HT-29 cells with an absorptive and mucus-secreting phenotype, respectively on transwell insert plates. Our Models come with media, and you can also order additional media. We provide a series of 3D organoid BBB model culture substrates, such as collagens and hydrogels, for you.	inquiry
BBM-M-006	Ready-to-Use Blood-Brain Barrier Modeling Kit	Ready-to-Use Blood-Brain Barrier Modeling Kit from Ace Therapeutics is developed by co-culturing three cell types, primary cultures of rat (Wistar rat) brain capillary endothelial cells, brain pericytes, and astrocytes, which is an in vitro reconstituted BBB model and shows many of the in vivo BBB features. Our Models come with media, and you can also order additional media. We provide a series of 3D organoid BBB model culture substrates, such as collagens and hydrogels, for you.	inquiry
BBM-M-007	Blood-Brain Barrier Organ-on-a-Chip	Blood-Brain Barrier Organ-on-a-Chip from Ace Therapeutics is established by replicating the blood-brain barrier (BBB), which is an excellent model for studying the interactions between cells and the transmission of materials and signals between cells. This Blood-Brain Barrier Organ-on-a-Chip's peripheral chambers are used to culture the first type of cells, while the central chamber is used to culture the second type of cells. The porous structure enables communication between cells in the peripheral compartment and cells in the central compartment.	inquiry
BBM-M-008	High-throughput Blood-Brain Barrier Organ-on-a-Chip (IBAC MI)	High-throughput Blood-Brain Barrier Organ-on-a-Chip (IBAC MI) from Ace Therapeutics is a high-throughput organ chip suitable for building the blood-brain barrier. It can not only monitor cells in real-time but also realize multi-organ co-culture.	inquiry
BBM-M-009	3D Blood Brain Barrier Model (TEER configuration)	3D Blood Brain Barrier Model recreates the in vivo microenvironment by mimicking the histology of brain tissue cells that communicate with BBB epithelial endothelial cells. The IMN2 TEER option allows you to accurately measure resistance, providing a non-invasive method to monitor tight junctions in real-time.	inquiry
BBM-M-010	3D Blood-Brain Barrier Model (IMN2 linear)	3D Blood Brain Barrier Model recreates the in vivo microenvironment by mimicking the histology of brain tissue cells that communicate with BBB epithelial endothelial cells.	inquiry
BBM-M-011	3D Blood-Brain Barrier Model (IMN2 radial)	3D Blood Brain Barrier Model recreates the in vivo microenvironment by mimicking the histology of brain tissue cells that communicate with BBB epithelial endothelial cells.	inquiry
BBM-M-012	Blood-Brain Barrier Organ Chip for Penetrance Test	Blood-Brain Barrier Organ Chip for Penetrance Test from Ace Therapeutics is an organ-on-a-chip consisting of tube chambers that represent blood circulation in the brain's microcirculation, allowing assessment of whether compounds can cross the BBB.	inquiry
BBM-M-013	Blood-Brain Barrier Plug-and-Play Instrument Pack	Blood-Brain Barrier Plug-and-Play Instrument Pack from Ace Therapeutics includes two channels, a vascular channel and a neural channel, separated by a porous membrane, simulating the BBB through dynamic culture and application of shear stress.	inquiry
BBM-M-014	Ready-To-Use High-Throughput Blood-Brain Barrier Organ-on-a-Chip with HBMEC Tubules	Ready-to-use High-throughput Blood-Brain Barrier Organ-on-a-Chip with HBMEC Tubules from Ace Therapeutics is a microfluidic chip that provides a rapid method to access blood-brain Data related to barrier studies.	inquiry

All of our products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.