In Vitro Pharmacology Services for Stroke Drugs

Currently, oxygen-glucose deprivation (OGD)-induced models are the most relevant and commonly used in vitro models to simulate stroke. Assays based on in vitro stroke models can be used to understand pathogenic mechanisms and drug discovery screening, effectively quantifying cytotoxicity, biological activity, biochemical mechanisms, and off-target interactions. Cell-based assays provide complex biologically relevant data and are valuable tools in stroke drug discovery applications. Unlike traditional biochemical assays, cell-based assays are more physiologically relevant and allow for simultaneous assessment of compound properties.

Custom In Vitro Pharmacology Services for Stroke Drugs

At Ace Therapeutics, we offer a comprehensive range of ischemic stroke in vitro assays to help our clients study study pathogenic mechanisms and drug discovery screening. Our ischemic stroke in vitro assays are based on nerve cell exposure under hypoxic-glucose deprivation conditions. Ischemic-like conditions are induced by replacing normal O₂/CO₂ balanced medium with N2/CO₂ balanced medium in a hypoxic chamber. We also exposed cells to OGD conditions using the glucose-free medium.

Our ischemic stroke in vitro assay service allows for robust, flexible, and customized analysis of drugs to be tested on a wide range of cell lines. At Ace Therapeutics, we want to help scientists in the field of stroke by offering a wide range of assays that measure or detect cellular events. We would be happy to help you choose the right assay!

• Cell Viability Assay
• Caspase-3 Activity Assay
• In Vitro Neuroinflammatory Models and Assays
• Excitotoxicity In Vitro Assay
• Cell-Based Mitochondrial Function Assay
• In Vitro Oxidative Stress Assay

Cell Lines Used

• Primary rodent neuronal cell or neuroblastoma cell lines, e.g. SH-SY5Y cell line.
• Human induced pluripotent stem cell (hiPSC)-derived neural cells.

Our Advantages and Features

• Our highly qualified research team is available to assist our clients in the development of new drugs for stroke and the exploration of drug mechanisms of action.
• We have established standard procedures for differentiating iPSC lines into a wide range of cell types in the neurological field. Cell lines can be customized upon request.
• With our off-the-shelf iPSC-derived models, you can conduct your research with consistency and reproducibility.
• The ischemic stroke in vitro assay accommodates high-throughput screening of stroke drugs.

After oxygen sugar deprivation, Ace Therapeutics offers several studies to analyze the different stages of the cell death process and the possible recovery of damaged neurons. By using multiple cell types and utilizing proven and innovative technologies, we can develop assays that can best mimic stroke to accelerate your stroke drug discovery program. If you are interested in our services, please do not hesitate to contact us!