Cell-Based Mitochondrial Function Assay Services for Stroke Drug Discovery

Introduction to the Mitochondrial Dysfunction in Stroke

Mitochondria are multifunctional organelles involved in many metabolic activities, including energy production and synthesis of biomolecules. Pathophysiological abnormalities transform mitochondria from vital organelles to activators of cell death. Mitochondria play a crucial role in ischemia-induced cell death by impairing ATP synthesis and participating in apoptotic and necrotic pathways. The calcium overload, opening of mitochondrial permeability transition pore, and excessive generation of reactive oxygen species (ROS) are mitochondrial pathology contributing to neuronal death after stroke. Mitochondria have been recognized as an urgent target to advance new therapeutic interventions for stroke.

Fig. 1. Mitochondrial dysfunction contributing to neuronal death after stroke. (Li et al., 2017)

Custom Cell-Based Measurement of Mitochondrial Function Services

Ace Therapeutics provides reliable cell-based assay services to assess the effects of stroke drugs on mitochondrial activity and mitochondria-associated cell death.Based on in vitro models of ischemic stroke, we offer multiple methods to examine the ability of stroke drugs to resist stroke-induced depolarization and reduction of mitochondrial membrane activity in vitro. We treat cells with stroke drugs and then add dyes to measure mitochondrial function in an automated fluorescent imaging platform. We finally compare the fluorescence signals of the test compounds to control samples. All studies can be customized to meet client needs.

• Mitochondrial membrane depolarization assay
• Mitochondrial superoxide generation detection
• Mitochondrial calcium ion detection
• Mitochondrial membrane permeability transition pore detection

Experimental Design

• Format: 96-well plate (customized formats available)
• Measurement: High-content image analysis
• Cells: A range of different neuronal cell models including immortalized neuroblastoma cells, primary mouse cortical neurons, and iPSC-derived excitatory neurons.

Why Choose Us

• We offer high-throughput cell assays for measuring mitochondrial function.
• Mitochondrial function assays can be performed on live cells or ex vivo animal tissues and offer the ability to multiplex with immunostaining or other fluorescence assays.
• Our advanced fluorescent probes, imaging systems, and flow cytometry platforms provide high-resolution data and enable precise quantification of mitochondrial parameters.
• Our efficient laboratory workflow, stringent quality control measures and expert program management ensure that our clients receive accurate and timely results.

Ace Therapeutics leverages advanced assays and technology, combined with customized experimental designs and expert data analysis, to evaluate the neuroprotective capabilities of stroke drugs by measuring mitochondrial function. We aim to accelerate stroke drug discovery and development. Please do not hesitate to contact us!

1. Li, Q., & Gao, S. (2017). Mitochondrial dysfunction in ischemic stroke. Translational research in stroke, 201-221.