Cell Viability Assay Services for Stroke Drug Discovery & Development

Cell viability is the number of healthy, living cells in a sample. Measurement of cell viability plays a fundamental role in all forms of cell culture. For in vitro models of stroke based on oxygen-glucose deprivation (OGD), 20-60% OGD-induced cell death is an ideal way to assess the neuroprotective effects of compounds. It can be used for toxicity analyses or to correlate cell behavior with cell numbers to accelerate stroke drug screening.

Fig. 1. Increased expression of caspase-3 in the subventricular zone and the dentate gyrus during the period of stroke recovery. Fig. 1. Application of PC12 NS-1 in vitro stroke model. (Chua et al., 2021)

Custom Cell Viability Assay Services

Ace Therapeutics uses OGD-induced models to mimic in vitro models of stroke , providing a range of cell viability assays to support your screening for any stroke drugs of interest. We offer multiple methods (such as MTT, CCK8, LDH, MTS, Resazurin, and ATP assay) to assay the effects of stroke drug candidates on cell viability (cytotoxicity) on a wide range of neuronal cells to accelerate drug screening.

Primary rodent neuronal cell or neuroblastoma cell lines
Human induced pluripotent stem cell (hiPSC)-derived neural cells

With our expertise, state-of-the-art facilities, customized assay development, and comprehensive data analysis capabilities, Ace Therapeutics offers a comprehensive range of cell viability assay services to support stroke drug discovery efforts and accelerate the development of novel therapies. If you are interested in our services, please do not hesitate to contact us!

Reference

Chua, P., & Lim, W. K. (2021). Optimization of a PC12 cell-based in vitro stroke model for screening neuroprotective agents. Scientific reports, 11(1), 8096.

All of our services are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.