In Vitro Oxidative Stress Assay Services for Stroke Drug Discovery & Development

Introduction to the Role of Oxidative Stress in Stroke

Reactive oxygen species (ROS) are a group of reactive oxygen-containing molecules that readily react with macromolecules, leading to irreversible functional changes or even complete destruction. Their concentrations are difficult to measure directly in the brain. ROS plays a crucial role in human physiological processes, but excess ROS produces oxidative stress, which is essential for the development of the cascade of ischemic injury. Oxidative stress has the potential to cause cell death during cerebral ischemia and ultimately brain death after reperfusion. In particular, during reperfusion, superoxide anion radicals, hydroxyl radicals, and nitric oxide (NO) are produced, which can cause lipid peroxidation, inflammation, and apoptosis.

Fig. 1. In vitro ischemia induces nitro-oxidative stress. (Tajes et al., 2013)

Custom In Vitro Oxidative Stress Assay

Based on the in vitro models of ischemic stroke induced with oxygen-glucose deprivation (OGD), Ace Therapeutics provides reliable oxidative stress assay service to analyze ROS production in neuronal cells after modeling ischemia stroke. Our oxidative stress assay identifies the changes in the oxidative state of neuronal cells in cultures under ischemic conditions and evaluates the ability of stroke drugs to resist stroke-induced oxidative stress.

Our laboratory has advanced fluorescence microscopy, flow cytometry and microplate analysis platforms to track different parameters of oxidative stress. Specific assays can be customized according to client needs.

• ROS measurement
• Catalase activity measurement
• Lipid peroxidation assay
• Redox membrane potential and glutathione reduction

Cell Line Used

We offer a wide selection of cell lines such as human neuroblastoma (SH-SY5Y), murine glial cells (BV2), human endothelial cell line (HUVEC), and human brain myoblasts (HC-VSMC) primary cultures.

Common Deliverables

• Cell culture and cell assays are performed in triplicate for each condition.
• Raw data (plate readings, graphs, etc.) are dependent on the desired test.
• Test results are analyzed
• Formal report

Ace Therapeutics offers a variety of simple and sensitive oxidative stress assays to assess the role of stroke drug candidates in counteracting the damaging effects of free radicals in order to accelerate our clients' stroke programs. Please contact our scientists to discuss further details!

1. Tajes, M., et al. (2013). Nitro-oxidative stress after neuronal ischemia induces protein nitrotyrosination and cell death. Oxidative Medicine and Cellular Longevity, 2013.