Cell Lines for BBB Modeling

As the most basic unit in biological research, cells have been widely used as the vital study tool of basic research, drug screening, disease models and tissue engineering, thereby providing an important evidence for drug development and treatment. Although traditional animal models can more comprehensively simulate the complexity and specificity of human diseases, they are not easy to conduct a large number of repeated experiments due to the 3R principle and animal ethics issues. Compared with animal models, cell models, as a simple, fast and economical research tool, provide researchers with more opportunities to initially explore the mechanisms of disease and conduct large-scale drug screening.

BBB Models

The blood-brain barrier (BBB) refers to an extremely important histological barrier located in the intima of cerebral blood vessels, which composed of special cells including astrocytes, vascular endothelial cells and pericytes to generate neurovascular units and thereby involved in the regulation of neuronal activity. With the advancement of science and technology, more and more cell lines are used to construct in vitro BBB models. There are several cell lines that are commonly used for modeling the BBB.

Brain capillary endothelial cell line (BCEC): The BCEC cell line is derived from brain capillary endothelial cells, which can form tight junctions and express various transporters and enzymes in the blood-brain barrier.
Brain microvascular endothelial cell line (BMEC): The BMEC cell line is derived from brain microvascular endothelial cells and has been shown to exhibit multiple characteristics of the blood-brain barrier and can be used alone to create BBB models, such as human brain microvascular endothelial cell line (HBMEC).
Astrocyte-derived cell lines: Astrocytes play a critical role in maintaining the integrity and function of the blood-brain barrier and are often used in combination with BCEC or BMEC cell lines to model the BBB.
Pericyte-derived cell lines: Pericytes are another important cell type in the blood-brain barrier and are also commonly used in combination with BCEC or BMEC cell lines to model the BBB.

Ace Therapeutics provides hundreds of species, different types of cell lines that can be used alone or in combination to construct the models of BBB to study the behavior, structure and function of the blood-brain barrier, as well as for the development of drug delivery and treatment strategies for neurological diseases.

In addition to single cell lines, we provide multiple 3D coculture BBB models to more accurately represent the BBB, which typically involve a combination of endothelial cells (BCECs or BMECs) with other supporting cells such as astrocytes or pericytes.

Click on the Links to Access Our Cell Lines for BBB Modeling:

Primary Cells Immortalized Cells Stem Cells

Cat.#	Name	Description	Price
BBM-C-137	Rabbit Cerebrovascular Pericytes Cells	Rabbit Cerebrovascular Pericytes from Ace Therapeutics are isolated from the brain tissue of a pathogen-free New Zealand White Rabbit. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.	inquiry
BBM-C-138	C57BL/6 Mouse Astrocytes	C57BL/6 Mouse Astrocytes from Ace Therapeutics are isolated from brain tissues of pathogen-free laboratory mice. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.	inquiry
BBM-C-139	CD1 Mouse Astrocytes	CD1 Mouse Astrocytes from Ace Therapeutics are isolated from postnatal day 2 mouse brains. MA is characterized by immunofluorescence with antibodies specific to GFAP. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.	inquiry
BBM-C-140	Mouse Astrocytes-hippocampal from CD1	Mouse Astrocytes-hippocampal from CD1 from Ace Therapeutics are isolated from postnatal day 2 mouse hippocampus and are cryopreserved as primary cultures. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.	inquiry
BBM-C-141	Mouse Astrocytes-midbrain from CD1	Mouse Astrocytes-midbrain from CD1 (MA-mb) are isolated from embryonic day 15 mouse midbrain. MA-mb are cryopreserved as primary cultures and delivered frozen.	inquiry
BBM-C-142	Mouse Astrocytes-brain stem from CD1	Mouse Astrocytes-brain stem from CD1 (MA-bs) are isolated from embryonic day 15 mouse brain stem. MA-bs are cryopreserved as primary cultures and delivered frozen.	inquiry
BBM-C-143	Mouse Astrocytes-cerebellar from CD1	Mouse Astrocytes-cerebellar from CD1 from Ace Therapeutics are isolated from a pathogen-free mouse cerebellar and are cryopreserved as primary cultures. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.	inquiry
BBM-C-144	Rat Astrocytes	Rat Astrocytes (RA) from Ace Therapeutics are isolated from postnatal day 2 rat brains and are cryopreserved as primary cultures. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.	inquiry
BBM-C-145	Wistar Rat Astrocytes	Characterization: 1. Cell Activity; – Recovery Viability ≥ 80%. 2. Cell Purity; – GFAP-positive cells ≥ 70%. – β-tubulin III- or doublecortin- (Dcx-) positive cells ≤ 10%. – GalC-positive cells ≤ 10%.	inquiry
BBM-C-146	Sprague-Dawly (SD) Rat Astrocytes	Characterization: 1. Cell Activity; – Recovery Viability ≥ 80%. 2. Cell Purity; – GFAP-positive cells ≥ 70%. – β-tubulin III- or doublecortin- (Dcx-) positive cells ≤ 10%. – GalC-positive cells ≤ 10%.	inquiry
BBM-C-147	Rat Astrocytes-midbrain	Rat astrocytes-midbrain (RA-mb) from Ace Therapeutics are isolated from CD® IGS rat midbrain and are cryopreserved at P0. RA-mb are characterized by immunofluorescence with antibodies specific to GFAP. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.	inquiry
BBM-C-148	Rat Astrocytes-adult	Rat Astrocytes-adult (RA-a) from Ace Therapeutics are isolated from adult CD® IGS rat brains and are cryopreserved after purification. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.	inquiry
BBM-C-149	Bovine Astrocytes	Bovine astrocytes from Ace Therapeutics are isolated from the bovine brain and are cryopreserved at passage one. Bovine astrocytes are characterized by immunofluorescence with antibody specific to GFAP. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.	inquiry
BBM-C-150	Rabbit Astrocytes	Rabbit Astrocytes from Ace Therapeutics are isolated from rabbit brains and are cryopreserved at passage one. Rabbit Astrocytes are characterized by immunofluorescence with antibody specific to GFAP. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.	inquiry
BBM-C-151	Immortalized Human Brain Microvascular Endothelial Cells - SV40	Immortalized Human Brain Microvascular Endothelial Cells- SV40 from Ace Therapeutics are a specialized cell line developed by immortalizing primary cells using SV40 Large T antigen. These cells have been extensively characterized and demonstrated to maintain the immune barrier properties of primary HBMECs. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.	inquiry

All of our products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.