Apoptotic Mechanism Analysis after Cerebral Ischemia

Apoptosis is a highly regulated, energy-dependent form of cell death. In contrast to necrosis, apoptosis deals with the dismantling of excess cells from the inside in an organized manner, thereby minimizing damage and destruction to neighboring cells. Many neurons in the ischemic hemidiaphragm or peri-infarct zone may undergo apoptosis after a few hours or days. There are two general pathways for the activation of apoptosis in stroke: the intrinsic pathway and the extrinsic pathway. Anti-apoptotic signaling restores dysfunctional neurons, whereas pro-apoptotic signaling leads to neuronal death, thus regulating the balance between pro- and anti-apoptotic signaling could be a potential therapeutic target.

Fig. 1. Signaling pathways of ischemia-induced apoptosis, including intrinsic (mitochondria-mediated), extrinsic (receptor-mediated), and caspase-independent pathways. (Zheng et al., 2003)

Our Services

Ace Therapeutics' highly skilled team of experts with extensive experience in the field of stroke, provides reliable services to analyze the mechanisms of apoptosis after ischemic stroke and help clients discover anti-apoptotic signaling as a potential therapeutic target for stroke.

We use multifaceted approaches to study apoptotic mechanisms after cerebral ischemia. Our approach combines in vitro and in vivo models of ischemic stroke with advanced molecular and cellular techniques to help clients study the complex signaling pathways involved in apoptosis, including but not limited to:

• Analysis of Extrinsic Apoptosis Pathways in Ischemic Stroke
• Analysis of Intrinsic Pathways in Ischemic Stroke
• Analysis of P53 Apoptotic Pathways in Ischemic Stroke
• Analysis of Notch Signaling and Neuronal Death in Stroke

Our Methods

• We provide TUNEL staining, Annexin V/PI double staining, and caspase activity assay to quantitatively analyze the proportion and degree of apoptosis and the activation of apoptotic pathways.
• By analyzing the expression changes of key proteins and genes in the signaling pathways of ischemia-induced apoptosis, we can help clients explore the molecular mechanism of apoptosis and provide clues for targeted interventions.
• Using genetically engineered animal models, we can further verify the role of apoptotic pathway in ischemic brain injury, and provide experimental basis for the discovery of drug targets and the development of new therapies.

Ace Therapeutics provides comprehensive molecular mechanism analysis services to help clients elucidate the key role of apoptosis in the pathogenesis of stroke. With leading analysis tools and rich collaboration experience, Ace Therapeutics can provide clients with high-quality experimental data to support their new drug development for stroke targeting apoptosis-related signaling pathways. If you are interested in our services, please do not hesitate to contact us!

1. Zheng, Z., et al. (2003). Cellular and molecular events underlying ischemia-induced neuronal apoptosis. Drug news & perspectives, 16(8), 497-503.