Analysis of p53 Apoptotic Pathways in Ischemic Stroke

In addition to extrinsic apoptosis pathway and intrinsic apoptosis pathway, another programmed cell death process is largely dependent on p53 in ischemic stroke. The tumor suppressor p53 regulates key cellular processes including cell cycle arrest, DNA repair, senescence, and apoptosis. A large number of in vitro and in vivo studies have demonstrated the role of p53 in neuronal apoptosis and its accumulation in apoptotic lesions after cerebral ischemia. Furthermore, genetic deletion and pharmacologic inhibition of p53 prevent neuronal injury and improve functional recovery after stroke. However, the specific mechanism of neuronal damage by p53 in ischemic stroke is unknown and requires further investigation. Understanding the mechanisms that mediate p53 regulation and brain damage after stroke may help identify targets for neuroprotective therapy.

Fig. 1. p53 activates both transcriptional and mitochondrial apoptotic programs after stroke. (Almeida et al., 2021)

Our Services

Ace Therapeutics provides comprehensive services to analyze the p53-mediated apoptotic pathways in stroke. Our team of highly skilled experts combines expertise from various fields (e.g. molecular biology, neuroscience, bioinformatics, and imaging) to analyze the complex mechanisms controlling p53-mediated apoptosis and help clients to develop stroke drugs that mitigate neuronal damage and improve functional recovery. Our expertise enables us to design and perform comprehensive analyses, ensuring reliable and accurate results.

Identification and Validation of p53 Apoptotic Pathways Related Targets for Stroke

We use gene expression profiling techniques (e.g., RNA sequencing, microarray analysis) to comprehensively assess the transcriptional changes induced by p53 activation in ischemic stroke, including Bax, IGF-binding protein-3, Fas, the p53-inducible genes (PIG's), reaper, Noxa, p53AIP1, and PUMA.

Analysis of p53 Apoptotic Pathways in Experimental Models of Stroke

We provide in vitro and animal models of ischemic stroke to help clients study the role of the p53-mediated apoptotic pathway.

• We utilize advanced imaging techniques such as immunofluorescence and confocal microscopy to determine the nuclear and mitochondrial transport of p53 and its interaction with other cellular components.
• We use immunoblotting and mass spectrometry techniques to analyze the phosphorylation status of p53.

Ace Therapeutics, with its advanced analysis tools and rich collaborative experience, can help iclients explore the specific role of the p53 apoptotic pathways in the pathogenesis of stroke and discover its potential as a therapeutic target. If you are interested in our services, please do not hesitate to contact us!

1. Almeida, A., et al. (2021). Mitochondrial-nuclear p53 trafficking controls neuronal susceptibility in stroke. IUBMB life, 73(3), 582-591.