Analysis of Extrinsic Apoptosis Pathways in Ischemic Stroke

The extrinsic apoptosis pathway is one of the major pathways of apoptosis and plays an important role in the pathogenesis of ischemic stroke. This pathway is mainly triggered by the activation of cell surface death receptors, which leads to the cascade activation of downstream effector proteins caspase-8 and caspase-3, inducing apoptosis. In addition, the extrinsic apoptosis pathway can be amplified by the mitochondria-dependent endogenous apoptotic pathway, exacerbating neuronal apoptosis. Intervention of the extrinsic apoptosis pathway is an important direction in the treatment of stroke. It has been shown that the use of drugs that inhibit death receptors or downstream caspases can significantly reduce ischemic brain injury and exert neuroprotective effects.

Fig. 1. Extrinsic and intrinsic pathways of apoptosis and related molecules in ischemic stroke. (Mohtasham et al., 2021)

Our Services

Ace Therapeutics provides comprehensive services for analyzing the extrinsic apoptosis pathway in ischemic stroke. Our services encompass a wide range of techniques to help clients study the molecular mechanisms of apoptosis and identify potential therapeutic targets.

Identification and Validation of Extrinsic Apoptosis Pathways Related Targets for Stroke

We use bioinformatics tools and computational models to examine the interactions between ligands such as TNF-α, FasL, and TRAIL, and their corresponding death receptors on the cell surface (TNF-α receptor 1, Fas/CD95/APO1, and TRAIL-R) to elucidate the binding kinetics, affinities, and downstream signaling events triggered by these interactions. With these data, we help clients to identify potential therapeutic targets related with extrinsic apoptosis pathway in ischemic stroke.

Analysis of Extrinsic Apoptosis Pathways in Experimental Stroke Models

We offer animal and in vitro models of ischemic stroke to help clients analyze the mechanisms of extrinsic pathways of apoptosis in ischemic injury and develop new stroke drugs that target apoptosis-related targets. We utilize advanced molecular analysis techniques to analyze the expression levels of death receptors, ligands, and key molecules involved in extrinsic pathways. We provide cell viability assays, TUNEL staining, and flow cytometry to assess the functional consequences of death receptor activation and caspase-mediated apoptosis.

Ace Therapeutics is committed to helping clients analyze the extrinsic apoptosis pathways in ischemic stroke and identify therapeutic targets. We strive to meet the needs of clients by providing them with valuable data to advance their research goals. If you are interested in our services, please do not hesitate to contact us!

1. Mohtasham, N., et al. (2021). Extrinsic and intrinsic pathways of apoptosis and related molecules in ischemic stroke. Central Asian J Med Pharm Sci Innovat, 1(4), 194-204.