Rat Primary Brain Microvascular Endothelial Cells (RPBECs) from Ace Therapeutics are isolated from the brain tissue of 1-day-old neonatal Sprague-Dawley rats. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Mouse Astrocytes-hippocampal from CD1 from Ace Therapeutics are isolated from postnatal day 2 mouse hippocampus and are cryopreserved as primary cultures. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.

Porcine Brain Microvascular Endothelial Cells from Ace Therapeutics are isolated from healthy porcine brain tissue. Porcine Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with a gelatin-based solution for 0.5 hours and incubated in Ace Therapeutics’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Rat Brain Vascular Pericytes (RBVP) from Ace Therapeutics are isolated from adult rat brains and cryopreserved at P2. MBVP expresses Neural/glial antigen 2 (NG2) and α-smooth muscle actin. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Characterization: 1. Cell Activity; – Recovery Viability ≥ 80%. 2. Cell Purity; – GFAP-positive cells ≥ 70%. – β-tubulin III- or doublecortin- (Dcx-) positive cells ≤ 10%. – GalC-positive cells ≤ 10%.

Human Astrocytes from Ace Therapeutics are isolated from the human cerebral cortex, cryopreserved at passage one. Each vial contains at least 5×10^5 cells per ml and is delivered frozen.

Rat Astrocytes-adult (RA-a) from Ace Therapeutics are isolated from adult CD® IGS rat brains and are cryopreserved after purification. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.

Mouse Astrocytes-midbrain from CD1 (MA-mb) are isolated from embryonic day 15 mouse midbrain. MA-mb are cryopreserved as primary cultures and delivered frozen.

C57BL/6 mouse brain endothelial cells (MBREC) from Ace Therapeutics are isolated from the brain tissue of pathogen-free laboratory mice. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Bovine astrocytes from Ace Therapeutics are isolated from the bovine brain and are cryopreserved at passage one. Bovine astrocytes are characterized by immunofluorescence with antibody specific to GFAP. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.