3D Psoriasis Model Development Services
At Ace Therapeutics, we specialize in providing advanced preclinical research services tailored for psoriasis research. Our 3D psoriasis model development services offer a sophisticated platform designed to assist researchers in understanding the complex mechanisms of psoriasis and evaluating potential therapeutic agents.
Introduction to 3D Psoriasis Models
3D psoriasis models not only provide a more accurate representation of the epidermal barrier but also incorporate the complex interplay between various cell types, including keratinocytes, fibroblasts, and immune cells. These models facilitate the investigation of cellular behavior, drug absorption, and the effects of therapeutic agents in a controlled environment that mimics in vivo conditions. Research has shown that 3D models exhibit key features of psoriatic skin, such as hyperproliferation of keratinocytes, altered lipid organization, and immune cell infiltration. For instance, studies have demonstrated that 3D psoriatic skin models can accurately reflect the dysregulated expression of cytokines and chemokines observed in psoriatic lesions. This level of detail enables researchers to investigate the underlying mechanisms of psoriasis pathology and assess the efficacy of new therapeutic agents in a controlled setting.
Fig. 1 Establishment of 3D human skin equivalents. (Scheurer J, et al., 2024)
Our 3D Psoriasis Model Development Services
At Ace Therapeutics, we specialize in developing custom 3D psoriasis models tailored to specific research needs. Our services encompass the entire process of model construction, from initial design to functional testing.
Custom Model Development
- Cell Line Selection: Utilizing a variety of skin-derived cell lines to create models that reflect the distinct characteristics of psoriatic skin.
- Layered Structure Formation: Constructing epidermal and dermal layers to accurately mimic human skin architecture.
Disease Characterization and Validation
- Histological Analysis: Utilizing techniques such as Masson's Trichrome and immunohistochemistry to assess the structural integrity and cellular composition of the models.
- Biochemical Assessments: Measuring key biomarkers of inflammation and keratinocyte activity to validate the model's disease representation.
- Functional Studies: Evaluating the barrier function and permeability of the skin models using transepidermal water loss (TEWL) measurements and diffusion studies.
Drug Testing and Evaluation
- Percutaneous Absorption Studies: Using Franz diffusion cells to measure the permeability of compounds through the skin model.
- Toxicity Assessments: Evaluating the cytotoxic effects of compounds on skin cells within the 3D model to provide essential safety data.
Our Technology Platform for 3D Psoriasis Model Development
Techniques
We use a self-assembly approach with primary human cells to develop 3D psoriasis models, preserving key features like lipid disorganization and keratinocyte proliferation.
Interface Culture
Our models implement an air-liquid interface culture to promote keratinocyte differentiation, closely mimicking the natural skin environment and enhancing physiological relevance.
Analytical Techniques
We perform functional assessments focusing on drug penetration and permeability, providing essential data to support therapeutic evaluations and drug development.
Ace Therapeutics is dedicated to providing high-quality, customizable models that meet the specific needs of researchers. For more information on our 3D psoriasis model development services or to discuss how we can support your research, please do not hesitate to contact us.
Reference
- Scheurer J, et al. Histological and functional characterization of 3D human skin models mimicking the inflammatory skin diseases psoriasis and atopic dermatitis. Dis Model Mech. 2024;17(1):dmm050541.