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Customized Rabbit Models of Corneal Neovascularization

The small size of the rodent eye limits the dose volume administered and may pose challenges for the evaluation of antivascular therapies. In order to meet the needs of different researchers, Ace Therapeutics provides two of the most commonly used rabbit corneal neovascularization models, the alkali burns corneal neovascularization model and the suture-induced corneal neovascularization model, to support their ongoing mechanistic research to prevent and treat CN.

Rabbit Models of Corneal Neovascularization

What Is Corneal Neovascularization?

The cornea, the transparent and avascular tissue, plays a vital role in refracting light and maintaining vision. The cornea's transparency depends on the balance between angiogenic and anti-angiogenic factors, and when this balance is disturbed, new blood vessels form on the cornea, ie, corneal neovascularization (NV). NV is the leading cause of blindness worldwide and is commonly seen in ocular surface diseases, chemical damage, corneal trauma, ocular surface diseases, and contact lens wear. Corneal opacity is due to the progressive invasion of existing blood vessels in the corneal NV, which eventually results in vision loss or disappearance.

Fig. 1. The corneal neovascularization area after alkali burns on day 7 and day 14 among the three subgroups. (Chen J, et al., 2021)

Service Overview

Corneal neovascularization is a vision-threatening disease, and the development of robust models of corneal neovascularization will help elucidate the mechanism of this disease. In order to meet various difficulties and challenges of ocular disease research, Ace Therapeutics ophthalmic pharmacologists have been committed to developing a full range of eye disease models. Over the past few years, Ace Therapeutics has explored various animal models of corneal neovascularization, including rats, mice, and rabbits. These models provide our clients with an opportunity to elucidate anti-angiogenic therapy mechanisms.

Explore Ace Therapeutics' Rabbit Models of Corneal Neovascularization

Ace Therapeutics' expert team can provide many common methods to construct NV models. In Ace Therapeutics laboratories, we offer two of the most commonly used rabbit corneal neovascularization models, the alkali burns corneal neovascularization model and the suture-induced corneal neovascularization model.

Establishment of an alkali burn corneal neovascularization model in rabbits

Our researchers place filter paper soaked in 1 mol/L NaOH solution on the corneal surface of anesthetized New Zealand rabbits at a distance of 2 mm from the upper limbus and burned them for 30 seconds to establish a model of corneal neovascularization in rabbits with alkali burns.

Establishment of suture-induced corneal neovascularization model in rabbits

Under rabbit anesthesia, our researchers induce a rabbit corneal neovascularization model by placing a single 7.0 silk suture at a mid-stromal depth approximately 1 mm from the limbus.

It is worth mentioning that we also provide a spontaneous corneal NV transgenic mouse model to help customers discover and study multiple intertwined signaling pathways that maintain corneal vascular supply. Please follow the link to access our rodent models of corneal neovascularization.

Available Support Services

In order to successfully construct the rabbit corneal neovascularization model, our scientists use a slit lamp examination to rule out any anterior segment disease before the model was established. In addition, to evaluate the development of corneal neovascularization and test anti-angiogenic therapy, Ace Therapeutics conduct a comprehensive evaluation, including but not limited to:

Ophthalmological examinations.
Clinical vascular assessment (slit-lamp biomicroscopy monitoring).
Histological analysis.
Immunohistochemical analysis.
Molecular biology analysis (evaluating candidate compounds' therapeutic mechanisms).

Ace Therapeutics aims to provide global customers with a powerful experimental tool to explore many aspects of ocular angiogenesis. This includes characterizing the complex interplay between various pro-angiogenic pathways and screening for potential anti-angiogenic molecules. If you are interested in our services or need more detailed information, please feel free to contact us. Our experienced scientists are ready to help you!

References

Chen J, Ding X, Du W, et al. Inhibition of corneal neovascularization by topical application of nintedanib in rabbit models. Int J Ophthalmol. 2021, 14(11):1666-1673.
Campos-Mollo E, Pérez-Santonja JJ, Lledó-Riquelme M, et al. New corneal neovascularization model in rabbits for angiogenesis research. Ophthalmic Res. 2011, 45(3):135-41.

For Research Use Only.

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