Customized In Vivo Ocular Models ace

Customized Rodent Models of Corneal Neovascularization

Corneal neovascularization (CN) is one of the most common eye diseases clinically. Ace Therapeutics provides a variety of corneal neovascularization model generation services to our global clients to support their ongoing mechanistic research to prevent and treat CN. We guarantee high standards and reliable services thanks to our advanced technology platform and experienced researchers. This will accelerate their ophthalmic preclinical research.

Rodent Models of Corneal Neovascularization

Corneal Neovascularization Model

Corneal Neovascularization (NV) refers to the growth of blood vessels and/or lymphatic vessels into the physiologically avascular cornea. The cornea has been the prime testing ground for anti-angiogenic strategies for decades due to its avascularity, accessibility, and visibility. Its avascularity provides an ideal system for assessing various factors' pro- and anti-angiogenic potential. In rodent models, corneal neovascularization models provide an efficient approach to discovering novel therapeutic strategies for angiogenesis-related ocular and extraocular diseases. Because of recent advances in treating ocular diseases with pathological angiogenesis, researchers have increasingly focused on developing reliable and reproducible models to study angiogenesis. Today, four general animal models of corneal neovascularization have been developed to study pathogenesis and evaluate novel anti-angiogenic drugs: the chemical injury model, corneal suture model, corneal micro pocket assay, and transgenic model.

Fig. 1. Principles for animal dry eye modeling. Fig. 1. Murine models of corneal angiogenesis. (Liu CH, et al., 2017)

Service Overview

Corneal neovascularization models provide a powerful tool for evaluating anti-angiogenic therapies. Over the past few years, Ace Therapeutics has developed various corneal neovascularization membrane models. This is based on its deep understanding of the critical mechanisms of corneal neovascularization. These models provide our customers with critical data on angiogenesis inhibitor properties for ophthalmic products.

Explore Ace Therapeutics' Rodent Models of Corneal Neovascularization

At Ace Therapeutics, our talented scientists are able to provide many commonly used methods for constructing NV models. We provide the following rodent corneal neovascularization models for global customers, including but not limited to:

Alkali burn corneal neovascularization model
Suture injured corneal neovascularization model
Mouse model corneal pocket assay

Ace Therapeutics researchers apply a specified amount of NaOH (1 N) aqueous solution-saturated filter paper disc to the center of the cornea for 30 seconds to construct a corneal neovascularization model. Alternatively, place one or more nylon sutures in the rat eye (corner stroma) to induce inflammation to form a corneal neovascularization model of suture injury. Alternatively, place the slow-release hydrogen polymer material of pro-angiogenic factors in the micro pouch of the mouse eye to generate an inflammatory response to form the mouse model corneal pocket assay.

Spontaneous corneal NV transgenic model

In addition to the above corneal neovascularization models induced by external stimuli, Ace Therapeutics also provides some genetically engineered mice with spontaneous corneal neovascularization to identify and study multiple intertwined signaling pathways that maintain corneal vascularity.

Tab. 1. Ace Therapeutics' Spontaneous Corneal NV Transgenic Mouse Model

Gene	Mouse model
Destrin	Corn1
Cd36	Cd36−/−
Pax6	Pax6Tg
KLEIP	KLEIP−/−
FoxC1	FoxC1+/-
Jam-A	Jam-A−/−
TSP-1	TSP-1−/−
LRIG1	LRIG1-/-

It is worth mentioning that our corneal neovascularization model is not limited to rodents. Our expert team has also developed a rabbit corneal neovascularization model. Contact our scientists for details.

Ace Therapeutics is committed to providing global customers with a powerful experimental tool to explore many aspects of ocular angiogenesis. This includes characterizing the complex interplay between various pro-angiogenic pathways and screening for potential anti-angiogenic molecules. If you are interested in our services or need more detailed information, please feel free to contact us. Our experienced scientists are ready to help you!

References

Liu CH, Wang Z, Sun Y, et al. Animal models of ocular angiogenesis: from development to pathologies. FASEB J. 2017 Nov;31(11):4665-4681.
Kather JN, Kroll J. Transgenic mouse models of corneal neovascularization: new perspectives for angiogenesis research. Invest Ophthalmol Vis Sci. 2014 Nov 25;55(11):7637-51.

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