TL1A-Responsive Luciferase Reporter Jurkat Cell Line

Product Details

Description	TNF-like ligand 1A (TL1A)-Responsive Luciferase Reporter Jurkat Cell Line is a TL1A-responsive DR3/NF-kB luciferase reporter Jurkat cell line expressing firefly luciferase under the control of an NF-kB response element, and with stable expression of human DR3 (death receptor 3; TNFRSF25; NM_003790.3). Expression of the firefly luciferase gene is driven by NF-kB response elements located upstream of the minimal TATA promoter. Activation of the NF-kB signaling pathway by the DR3 ligand TL1A (TNF-like Protein 1A; TNFSF15) can be monitored by measuring luciferase activity. This cell line has been validated to be responsive to TL1A and TNF-α stimulation. TL1A stimulation was blocked by the neutralizing anti-TL1A antibody.
Host Cell Line	Jurkat cells (clone E6-1), Human T lymphoblast, suspension
Applications	• Characterize soluble TL1A activity. • Screen anti-TL1A antibodies in a cell-based assay format.
Supplied As	Each vial contains >1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 2 Materials Required for Cellular Assay • TL1A, His-Tag, Avi-Tag Recombinant • Anti-TL1A Antibody • Growth Medium 2A • Assay Medium: Thaw Medium 2 • 96-well tissue culture treated, white, clear-bottom assay plate • Luminometer
UniProt	TL1A: O95150; DR3: Q93038
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only