TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line

Product Details

Synonyms	CRISPR, TCR knockout, CAR-T, killing assay
Description	TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line is a Jurkat cell line where TCR (T Cell Receptor) was knockout. The TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from Jurkat cells stably expressing the firefly luciferase reporter under the control of NFAT response elements. This cell line has been functionally validated and does not respond to anti-CD3 agonist antibodies, as opposed to the parental NFAT-Luciferase Reporter Jurkat cells.
Host Cell Line	Jurkat (clone E6-1), human T lymphoblast, suspension
Applications	Use as a control to determine CAR-T-specific cell killing
Supplied As	Each vial contains ˃1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 2  • Growth Medium 2B Materials Used in the Cellular Assay • Thaw Medium 2  • NFAT-Luciferase Reporter Jurkat cells • Anti-CD3 Agonist Antibody • Luminometer • 96-well tissue culture plate, white, clear bottom
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Immunotherapy, CAR-T

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only