TCF/LEF Reporter - HEK293 Cell Line (Wnt Signaling Pathway)

Product Details

Synonyms	WNT reporter, TCF cell line, LEF cell line, luciferase reporter, TCF/LEF Reporter
Description	The TCF/LEF Reporter – HEK293 Cell Line (Wnt Signaling Pathway) contains a firefly luciferase gene under the control of TCF/LEF responsive elements stably integrated into HEK293 cells. This cell line is validated for the response to the stimulation of mouse and human Wnt3a and to the treatment with an inhibitor of Wnt/ß-catenin signaling pathway.
Host Cell Line	HEK293 cells
Applications	• Monitor Wnt signaling pathway activity • Screen for compound activity of the Wnt/β-catenin signaling pathway
Supplied As	Each vial contains ~2 X 106 cells in 1 mL of 10% DMSO
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 1 • Growth Medium 1B Materials Required for Cellular Assay • LiCl • Mouse Wnt3a or human Wnt3a • IWR-1-endo: inhibitor of Wnt pathway • Assay Medium: Thaw Medium 1 • Growth Medium 1B • 96-well tissue culture treated white clear-bottom assay plate • Luminometer
UniProt	Q9UJU2
Mycoplasma Testing	The cell line has been screened using the PCR-based Mycoplasma Detection kit to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway, WNT

Storage & Handling

Storage/Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only