STAT5 Luciferase Reporter U937 Cell Line

Product Details

Synonyms	Signal Transducer And Activator Of Transcription 5A cell line, MGF cells, U937 Reporter
Description	The STAT5 (Signal transducer and activator of transcription 5) Luciferase Reporter U937 cell line monitors STAT5-mediated signal transduction pathways in the human monocyte cell line U937. It contains a firefly luciferase gene driven by STAT5 response elements located upstream of the minimal TATA promoter. After activation by GM-CSF (Granulocyte-macrophage colony-stimulating factor), the endogenous transcription factor STAT5 binds to the response elements, inducing transcription of the luciferase reporter gene. These cells respond to GM-CSF. Functional validation experiments showed that GM-CSF-induced luciferase activity was inhibited by anti-GM-CSF neutralizing antibody (Clone #BVD2-21C11).
Host Cell Line	U937
Applications	• Monitor STAT5 activity. • Screen for compounds regulating the STAT5 signaling pathway. • Study the effect of GM-CSF neutralizing antibodies on STAT5 activity.
Supplied As	Each vial contains ~ 2 x 106 cells in 1 mL of 10% DMSO in FBS
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 8 • Growth Medium 8A Materials Required for Cellular Assay • Recombinant Human GM-CSF • Anti-GM-CSF neutralizing antibody • White, clear-bottom 96-well tissue culture plate • Luminometer
UniProt	P42229
Mycoplasma Testing	The cell line has been screened using the Mycoplasma Detection kit to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only