STAT3 Reporter Jurkat Cell Line

Product Details

Synonyms	Signal transducer and activator of transcription 3, Acute-phase response factor, APRF
Description	The STAT3 Reporter Jurkat cell line is designed for monitoring the STAT3 signal transduction pathway. It contains a firefly luciferase gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines or growth factors that act on the receptor, endogenous STAT3 translocate to the nucleus and binds to the DNA response elements, inducing transcription of the luciferase reporter. This cell line responds to human interferons IFN-α and IFN-γ but not to interleukins IL-6, IL-1α, IL-2, or TNF-α. Activation of the STAT3 pathway by IFN-α is inhibited by JAK inhibitor CP 690,550.
Host Cell Line	Jurkat (clone E6-1), human T lymphoblast, suspension
Applications	Screen compound activity on the STAT3 signaling pathway.
Supplied As	Each vial contains > 1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 2 • Growth Medium 2K Materials Required for Cellular Assay • Thaw Medium 2 • Human IFN-alpha A • Human IFN-γ • IL-6 • IL-1α • IL-2 • TNF-α • Pan-JAK inhibitor CP 690,550 • Luminometer
UniProt	P40763
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only