STAT3 Luciferase Reporter THP-1 Cell Line

Product Details

Synonyms	Signal transducer and activator of transcription 3, Acute-phase response factor, APRF
Description	The STAT3 Luciferase Reporter THP-1 Cell Line monitors the STAT3 (Signal Transducer and Activator of Transcription 3) signal transduction pathway. It contains a firefly luciferase reporter driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, the endogenous transcription factor STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene. These cells respond to IL-6 (Interleukin 6), to G-CSF (Granulocyte Colony-Stimulating Factor) and to IFN-γ (Interferon γ).
Host Cell Line	THP-1, monocyte, suspension
Applications	• Monitor STAT3 activity in a monocyte cell line (THP1). • Screen for effects of compounds on the STAT3 signaling pathway. • Screen for inhibitors of STAT3-mediated IL-6 signaling.
Supplied As	Each vial contains >1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 8 • Growth Medium 8A Materials Required for Cellular Assay • Thaw Medium 8 • Recombinant Human IL-6 Protein • IFN-γ  • Pan-JAK inhibitor CP 690,550 • Human IL-6R alpha Antibody • Recombinant Human G-CSF Protein • Anti-GCSFR Antibody • White, clear-bottom cell culture plate, 96-well • Luminometer
UniProt	P40763
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only