STAT3 Luciferase Reporter HEK293 Cell Line

Product Details

Synonyms	Acute-Phase Response Factor, DNA-Binding Protein APRF, ADMIO, Signal Transducer And Activator Of Transcription 3
Description	The STAT3 Luciferase Reporter HEK293 Cell Line is a HEK293 cell lines expressing firefly luciferase driven by STAT3 response elements located upstream of a minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT3 binds to the response elements, inducing transcription of the luciferase reporter. This cell line has been validated to respond to human interleukin-6 (IL-6) and human IL-27. Functional validation experiments showed that IL-6-induced luciferase activity was decreased by the JAK inhibitor CP 690,550 and by an anti-IL-6 Receptor neutralizing antibody.
Applications	• Monitor STAT3 activity. • Screen for compound effect on the STAT3 signaling pathway. • Screen for inhibitors of STAT3-mediated IL-6 signaling.
Supplied As	Each vial contains >1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 1 • Growth Medium 1N Materials Required for Cellular Assay • Human IL-6 • JAK inhibitor CP 690,550 • Anti-IL-6R anitbody • Assay Medium: Thaw Medium 1 • Growth Medium 1N • 96-well tissue culture treated white clear-bottom assay plate • Luminometer
UniProt	P40763
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only