Cat. No.: IBDRGCL60479
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| Synonyms | SRE cell line, MAPK reporter, ERK reporter |
| Description | The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylates and activates ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes. The SRE Reporter – HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line. This cell line is validated for the response to the stimulation of EGF or serum and to the treatment of inhibitors of ERK signaling pathway. |
| Host Cell Line | HEK293 |
| Applications | • Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity. • Screen for activators or inhibitors of the MAPK/ERK signaling pathway. |
| Supplied As | Each vial contains ~1.5 X 106 cells in 1 mL of 10% DMSO |
| Materials Required But Not Supplied | Materials Required for Cell Culture • Thaw Medium 1 • Growth Medium 1B Materials Required for Cellular Assay • Thaw Medium 1 • Growth Medium 1B • Recombinant human EGF • U0126: Inhibitor of ERK pathway (MEK inhibitor). Prepare stock solution of U0126 in DMSO. • Assay Medium 1B • 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate • Luminometer |
| UniProt | P27361, P28482 |
| Mycoplasma Testing | The cell line has been screened using the PCR-based Mycoplasma Detection kit to confirm the absence of Mycoplasma species. |
| Scientific Category | Cell Signaling Pathway |
Storage & Handling
| Storage/Stability | Store in liquid nitrogen immediately upon receipt. |
| Shipping Temperature | -80°C |
| Warnings | Avoid freeze/thaw cycles |
| Regulatory Status | Research Use Only |