SRE eGFP Reporter - HEK293 Cell Line (ERK Pathway)

Product Details

Synonyms	SRE cell line, MAPK reporter, ERK reporter
Description	Recombinant HEK-293 cells expressing enhanced green fluorescent protein (eGFP) under the control of SRE responsive elements. This cell line is validated for its response to EGF or serum stimulation and to treatment with inhibitors of ERK signaling pathway.
Host Cell Line	HEK293
Applications	• Screen for compound effect on the MAPK/ERK signaling pathway. • Monitor MAPK/ERK signaling pathway activity and SRF-mediated activity.
Supplied As	2 vials: Each vial contains 2 x 106 cells in 1 mL of 90% FBS, 10% DMSO
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 1 • Growth Medium 1J Materials Required for Cellular Assay • Thaw Medium 1 • Growth Medium 1B • Assay Medium 1B • Recombinant human EGF • U0129 (MEK inhibitor) • 24-Well Tissue Culture Treated Plate • Round-Bottom 96 well plates or Round-Bottom Tubes • Flow Cytometry Cell Staining Buffer • Flow Cytometer or Fluorescence Microscope
UniProt	P27361, P28482
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles. Do not use a -80°C freezer for long term storage.
Regulatory Status	Research Use Only