Recombinant Human NLRP3 (mutated K232A) Protein

Target Information

Sequence	KMASTRCKLA RYLEDLEDVD LKKFKMHLED YPPQKGCIPL PRGQTEKADH VDLATLMIDF NGEEKAWAMA VWIFAAINRR DLYEKAKRDE PKWGSDNARV SNPTVICQED SIEEEWMGLL EYLSRISICK MKKDYRKKYR KYVRSRFQCI EDRNARLGES VSLNKRYTRL RLIKEHRSQQ EREQELLAIG KTKTCESPVS PIKMELLFDP DDEHSEPVHT VVFQGAAGIG KTILARKMML DWASGTLYQD RFDYLFYIHC REVSLVTQRS LGDLIMSCCP DPNPPIHKIV RKPSRILFLM DGFDELQGAF DEHIGPLCTD WQKAERGDIL LSSLIRKKLL PEASLLITTR PVALEKLQHL LDHPRHVEIL GFSEAKRKEY FFKYFSDEAQ ARAAFSLIQE NEVLFTMCFI PLVCWIVCTG LKQQMESGKS LAQTSKTTTA VYVFFLSSLL QPRGGSQEHG LCAHLWGLCS LAADGIWNQK ILFEESDLRN HGLQKADVSA FLRMNLFQKE VDCEKFYSFI HMTFQEFFAA MYYLLEEEKE GRTNVPGSRL KLPSRDVTVL LENYGKFEKG YLIFVVRFLF GLVNQERTSY LEKKLSCKIS QQIRLELLKW IEVKAKAKKL QIQPSQLELF YCLYEMQEED FVQRAMDYFP KIEINLSTRM DHMVSSFCIE NCHRVESLSL GFLHNMPKEE EEEEKEGRHL DMVQCVLPSS SHAACSHGLV NSHLTSSFCR GLFSVLSTSQ SLTELDLSDN SLGDPGMRVL CETLQHPGCN IRRLWLGRCG LSHECCFDIS LVLSSNQKLV ELDLSDNALG DFGIRLLCVG LKHLLCNLKK LWLVSCCLTS ACCQDLASVL STSHSLTRLY VGENALGDSG VAILCEKAKN PQCNLQKLGL VNSGLTSVCC SALSSVLSTN QNLTHLYLRG NTLGDKGIKL LCEGLLHPDC KLQVLELDNC NLTSHCCWDL STLLTSSQSL RKLSLGNNDL GDLGVMMFCE VLKQQSCLLQ NLGLSEMYFN YETKSALETL QEEKPELTVV FEPSW
Sequence Similarities	Belongs to the NLRP family. Contains 1 DAPIN domain. Contains 9 LRR (leucine-rich) repeats. Contains 1 NACHT domain.
Amino Acids	2 to 1036
Cellular Localization	Cytoplasm.
Tissue Specificity	Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
Function	May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.

Product Details

Product Type	Protein
Species	Human
Source	Baculovirus Infected Sf9 Cells
Tag	His-DDDDK Tag
Modifications	mutated K232A
Protein Length	Full length protein
Molecular Weight	120 kDa
Purity	≥56%
Active	No
Animal free	No
Nature	Recombinant
Application	SDS-PAGE

Storage & Handling

Shipping	Shipped with Dry Ice.
Storage	Store at -80 °C.
Handling	Avoid freeze / thaw cycle.