Recombinant Human ErbB4 / HER4 Protein

Target Information

Sequence	QSVCAGTENKLSSLSDLEQQYRALRKYYENCEVVMGNLEITSIEHNRDLS FLRSVREVTG YVLVALNQFRYLPLENLRIIRGTKLYEDRYALAIFLNY RKDGNFGLQELGLKNLTEILNG GVYVDQNKFLCYADTIHWQDIVRNPW PSNLTLVSTNGSSGCGRCHKSCTGRCWGPTENHC QTLTRTVCAEQCDG RCYGPYVSDCCHRECAGGCSGPKDTDCFACMNFNDSGACVTQCPQT FV YNPTTFQLEHNFNAKYTYGAFCVKKCPHNFVVDSSSCVRACPSSKMEVEE NGIKMCKP CTDICPKACDGIGTGSLMSAQTVDSSNIDKFINCTKINGN LIFLVTGIHGDPYNAIEAID PEKLNVFRTVREITGFLNIQSWPPNMTD FSVFSNLVTIGGRVLYSGLSLLILKQQGITSL QFQSLKEISAGNIYIT DNSNLCYYHTINWTTLFSTINQRIVIRDNRKAENCTAEGMVCNH LCSS DGCWGPGPDQCLSCRRFSRGRICIESCNLYDGEFREFENGSICVECDPQC EKMEDG LLTCHGPGPDNCTKCSHFKDGPNCVEKCPDGLQGANSFIFKY ADPDRECHPCHPNCTQGC NGPTSHDCIYYPWTGHSTLPQHARTP
Sequence Similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. Contains 1 protein kinase domain.
Amino Acids	26 to 651
Cellular Localization	Membrane and Nucleus. Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus.
Tissue Specificity	Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.
Function	Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. The C-terminal fragment (CTF) of isoform JMA-A CYT-2 (containing E4ICD2) can stimulate transcription in the presence of YAP1. ERBB4 intracellular domain is involved in the regulation of cell growth. Conflicting reports are likely due at least in part to the opposing effects of the isoform-specific and nuclear-translocated ERBB4 intracellular domains (E4ICD1 and E4ICD2). Overexpression studies in epithelium show growth inhibition using E4ICD1 and increased proliferation using E4ICD2. E4ICD2 has greater in vitro kinase activity than E4ICD1. The kinase activity is required for the nuclear translocation of E4ICD2.

Product Details

Product Type	Protein
Species	Human
Source	HEK 293 Cells
Tag	His
Endotoxin Level	<1.0 Eu/µg
Protein Length	Protein fragment
Molecular Weight	71 kDa
Purity	>95%
Active	Yes
Animal free	No
Nature	Recombinant
Application	FuncS, SDS-PAGE

Storage & Handling

Shipping	Shipped at 4 °C.
Storage	Store at -20 °C.
Handling	Avoid freeze / thaw cycle.