RARγ Luciferase Reporter HEK293 Cell Line

Product Details

Synonyms	RAR gamma, Retinoic acid receptor gamma, RARG
Description	RARγ Luciferase Reporter HEK293 Cell Line is a HEK293 cell line engineered to express firefly luciferase, under the control of retinoic acid response elements (RARE), and with full length human RARγ (retinoic acid receptor gamma) (accession # P13631-1). RARγ Luciferase Reporter HEK293 Cell Line is designed for monitoring the activity of RARγ. This cell line was functionally validated by stimulation with all-trans retinoic acid and Western Blot analysis.
Host Cell Line	HEK293, Human Embryonic Kidney, epithelial-like cells, adherent
Applications	• Monitor RARγ-regulated pathway activity. • Screen for agonists or antagonists of RARγ.
Supplied As	Each vial contains >1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 6 • Growth Medium 6A Materials Used in the Cellular Assay • ATRA • Assay Medium 6A • 96-well tissue culture treated white clear-bottom assay plate Luminometer
Biological Activity	This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid. The expression of RARγ is confirmed by Western blotting.
UniProt	P13631-1
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Nuclear Receptor

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only