Porcine RANTES ELISA Kit

Overview

Sample Type	Cell culture supernatant, Serum, Plasma
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard assay
Product Type	Chemokines
Sensitivity	46.9 pg/ml
Range	46.88 pg/ml - 3000 pg/ml
Species Reactivity	Reacts with: Pig
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
Tissue Specificity	T-cell and macrophage specific.
Sequence Similarities	Belongs to the intercrine beta (chemokine CC) family.
Cellular Localization	Secreted.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	Constituent: 100% PBS