Phospho-MSK1 (S376) ELISA Kit

Overview

Sample Type	Cell Lysate
Detection Method	Colorimetric
Assay Type	Semi-quantitative
Assay Duration	Multiple steps standard assay
Product Type	Redox Metabolism
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
Tissue Specificity	Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
Sequence Similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 protein kinase domains.
Cellular Localization	Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	Constituent: 100% PBS