Phospho-IKB alpha (S32) and Total IKB alpha ELISA Kit

Overview

Sample Type	Cell Lysate
Detection Method	Colorimetric
Assay Type	Semi-quantitative
Assay Duration	Multiple steps standard assay
Product Type	TLR Signaling
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Sequence Similarities	Belongs to the NF-kappa-B inhibitor family. Contains 5 ANK repeats.
Cellular Localization	Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	pH: 7.2 Constituent: 100% PBS