PD-1 (Mouse) / NFAT - Reporter - Jurkat Recombinant Cell Line

Product Details

Synonyms	Programmed Cell Death 1, PDCD1, SLEB2, CD279
Description	Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of mouse PD-1 (Programmed Cell Death 1, PDCD1, SLEB2, CD279, GenBank Accession #NM_008798).
Host Cell Line	Jurkat T Cell
Supplied As	Each vial contains 2 x 106 cells in 1 mL of 90% FBS, 10% DMSO
Materials Required But Not Supplied	• Mouse PD-L1/TCR activator-CHO cell line • Assay medium: Thaw Medium 2 • Growth Medium 2F • Anti-mouse PD-1 neutralizing antibody • Anti- PD-L1 neutralizing antibody • 96-well tissue culture-treated white clear-bottom assay plate • Luminometer • Thaw Medium 3 : Ham’s F-12 medium supplemented with 10% FBS, 1%Penicillin/Streptomycin.
UniProt	Q02242
Mycoplasma Testing	The cell line has been screened using the PCR-based Mycoplasma Detection kit to confirm the absence of Mycoplasma species.
Scientific Category	Immunotherapy

Storage & Handling

Storage/Stability	Immediately upon receipt, store in liquid nitrogen.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only