Notch1/CSL Luciferase Reporter HEK293 Cell Line

Product Details

Synonyms	Notch, NICD, PKA, cAMP, luciferase reporter
Description	The Notch1/CSL Luciferase Reporter HEK293 Cell Line is a HEK293 cell line that expresses the firefly luciferase reporter under the control of Notch-response elements (CSL responsive elements) and expression of Notch1DE (Notch1 that has a deletion of the entire extracellular domain). Inside the cells, the Notch1DE can be cleaved by γ-secretase. This active Notch1 NICD (Notch intracellular domain) translocated to the nucleus and induces the expression of the luciferase reporter. The cell line is validated for the inhibition of the expression of luciferase reporter using a known inhibitor of the Notch signaling pathway.
Host Cell Line	HEK293, Human Embryonic Kidney, epithelial-like cells, adherent
Applications	• Monitor Notch signaling pathway activity. • Screen for inhibitors of the Notch signaling pathway in a cellular model.
Supplied As	Each vial contains >1 x 106 cells in 1 mL of cell freezing medium)
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 1 • Growth Medium 1A Materials Required for Cellular Assays • DAPT • CSL Reporter HEK293 Cell Line • Thaw Medium 1 • 96-well tissue culture-treated white clear-bottom assay plate • Luminometer
Biological Activity	The cell line is validated for the inhibition of the expression of luciferase reporter using a known inhibitor of the Notch signaling pathway.
UniProt	P46531
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Store in liquid nitrogen immediately upon receipt.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only