NFAT Reporter (Luciferase) – THP-1 Cell Line

Product Details

Synonyms	nuclear factor of activated T cells, T cells transcription factor, NFAT1, NFATP, NFAT2; NFATc, NFATC1, NFATC2
Description	The NFAT reporter (Luciferase)-THP-1 cell line is designed for monitoring the NFAT (nuclear factor of activated T-cells) signaling pathway in THP-1 cells by measuring luciferase activity. It contains a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter. Upon activation by NFAT activators such as Ionomycin, endogenous NFAT transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.
Host Cell Line	THP-1 Human leukemia monocytic cell line. Non-adherent cells.
Applications	• Monitor NFAT signaling pathway activity. • Screen for compound activity on the NFAT signaling pathway.
Supplied As	2 vials: Each vial contains >1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 2 • Growth Medium 2M Materials Required for Cellular Assay • Ionomycin • PMA • Cyclosporin A • Assay Medium: Thaw Medium 2 • Growth Medium 2M • 96-well tissue culture treated white clear-bottom assay plate • Luminometer
Mycoplasma Testing	The cell line has been screened using the PCR-based Mycoplasma Detection kit to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only