NFAT Luciferase Reporter Jurkat Cell Line

Product Details

Synonyms	Nuclear factor of activated T cells; T cell
Description	The NFAT Luciferase Reporter Jurkat Cell Line expresses firefly luciferase under the control of the NFAT response elements stably integrated into the Jurkat cell genome. This reporter cell line is designed to monitor T cell activation as well as inhibition by various immune checkpoint inhibitors; it can be used as a control or parental cell line to co-express various immune checkpoint inhibitors, such as PD-1.
Host Cell Line	Jurkat (clone E6-1), human T lymphoblast, suspension
Applications	• Screen for compound activity on the PKC/ Ca2+ • Screen for agonists or antagonists of T cell receptors. • Control cell line when using immune checkpoint NFAT reporter cell lines. • Determine T cell activation through T cell receptor (TCR). • Analyze the functional activity of bispecific antibodies, such as Bispecific T Cell Engagers.
Supplied As	Each vial contains >1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 2 • Growth Medium 2B Materials Required for Cellular Assays Described in the Functional Validation section • Ionomycin • Phorbol 12-myristate 13-acetate (PMA) • TCR Activator CHO Recombinant Cell Line • Thapsigargin • Anti-CD19 x anti-CD3 bispecific antibody  • Anti-CD3 antibody • Anti-CD28 antibody • GSK-7975A • White, clear-bottom cell culture plate, 96-well • Luminometer
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Immunotherapy

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only