NF- κB Reporter (Luc) – THP-1 Cell Line

Product Details

Synonyms	NF-kB reporter, NFkappaB reporter, luciferase reporter, NF-kB reporter, NFkB reporter, nfkb thp1, thp1, NF-kB, nfkb, nf-kb
Description	The NF-κB reporter (Luc)-THP-1 cell line is designed for monitoring NF-κB (nuclear factor κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or NOD agonists, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.
Host Cell Line	THP-1 Human leukemia monocytic cell line. Non-adherent cells
Applications	• Monitor NF- κB signaling pathway activity. • Screen for compound activity on the NF-κB signaling pathway.
Supplied As	Each vial contains ~4 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 8 • Growth Medium 8A Materials Required for Cellular Assay • Recombinant Human hTNFα • LPS-EK (TLR4 and TLR2 agonist) • L18-MDP (NOD2 agonist) • IKK-16 dihydrochloride  • Assay Medium: Thaw Medium 8 • 96-well tissue culture-treated white clear-bottom assay plate • Luminometer
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only