NF-κB Reporter (Luc) – NIH/3T3 Cell line

Product Details

Synonyms	NF-kB reporter, NFkappaB reporter, luciferase reporter, NF-kB reporter, NFkB reporter, nih3t3, nfkb, nf-kb
Description	The NF-κB reporter (Luc)-NIH-3T3 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.
Host Cell Line	NIH/3T3
Applications	• Monitor NF-κB signaling pathway activity. • Screen for compound activity of the NF-κB signaling pathway.
Supplied As	Each vial contains ~2 x 106 cells in 1 mL of 10% DMSO
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 5 • Growth Medium 5A Materials Required for Cellular Assay • hTNFα • QNZ (EVP4593) or other suitable inhibitor control • Assay Medium: Thaw Medium 5 • Growth Medium 5A • 96-well tissue culture treated white clear-bottom assay plate • Luminometer
Mycoplasma Testing	The cell line has been screened using the PCR-based Mycoplasma Detection kit to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Immediately upon receipt, store in liquid nitrogen.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only