NF-κB Reporter (Luc) - HEK293 Recombinant Cell line

Product Details

Synonyms	NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, nfkb, nf-kb, TNFa reporter, IL-17 reporter, IL-1b reporter
Description	The NF-κB reporter (Luc) HEK293 cell line is designed to monitor nuclear factor Kappa B (NF-κB) activity. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or agonists of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. The cell line has been functionally validated in response to human TNF-α, IL-1β, and IL-17.
Host Cell Line	HEK293, epithelial-like cells, adherent
Species	Human
Applications	• Monitor NF-κB activity • Screen for compound activity on the NF-κB signaling pathway
Supplied As	Each vial contains 2 x 106 cells in 1 ml of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 1 • Growth Medium 1C Materials Used in the Cellular Assay • hTNFα • IL-1β • Assay Medium: Thaw Medium 1 • IKK-16 dihydrochloride: inhibitor of NF-κB activation • Anti-IL-17A Neutralizing Antibody • 96-well tissue culture-treated white clear-bottom assay plate • Luminometer
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells will arrive in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only