NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line

Product Details

Synonyms	NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, nfkb, nf-kb
Description	The NF-κB reporter (Luc)-HCT-116 cell line is designed to monitor nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or agonists of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.
Host Cell Line	HCT-116
Applications	• Monitor NF-κB signaling pathway activity in response to stimulants such as the cytokines TNFα and IL-1β, pathogen-associated molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern (DAMP) molecules (i.e. NOD1 ligand) (see references). • Screen for compound activity of the NF-κB signaling pathway
Supplied As	Each vial contains ~2 X 106 cells in 1 mL of 90% FBS, 10% DMSO
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 7 • Growth Medium 7A Materials Required for Cellular Assay • hTNFα • Assay Medium: Thaw Medium 7 • IKK-16 dihydrochloride: inhibitor of NF-κB activation • 96-well tissue culture-treated white clear-bottom assay plate • Luminometer
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Store in liquid nitrogen immediately upon receipt.
Shipping Temperature	dry ice
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only