NF-κB Luciferase Reporter Jurkat Cell Line

Product Details

Synonyms	nfkb, nf-kb reporter, nfkb report, nf-kb jurkat, nfkb, nf-kb
Description	The NF-κB Luciferase Reporter Jurkat Cell Line is a Jurkat cell line designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase reporter driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter. This cell line has been functionally validated and responds to phorbol 12-myristate 13-acetate (PMA) + ionomycin and TNFα. It can be used to measure TCR-mediated T cell activation through co-stimulation with anti-CD3 and anti-CD28 antibodies.
Host Cell Line	Jurkat (clone E6-1), human T lymphoblast, suspension
Applications	• Screen for compound activity on the NF-κB pathway. • Monitor NF-κB signaling pathway activity. • Determine T cell activation through T cell receptor.
Supplied As	Each vial contains 2 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 2 • Growth Medium 2B Materials Required for Cellular Assays Described in the Functional Validation section • Ionomycin • Phorbol 12-myristate 13-acetate (PMA) • TNFα • Anti-CD3 antibody • Anti-CD28 antibody • White, clear-bottom cell culture plate, 96-well • Luminometer
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Immunotherapy

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only